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Caspase-1 fluorometric assay kit

 
ALX-850-212-KI01 25 tests 261.00 USD
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Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate YVAD-AFC.

Product Details

Applications:Fluorescent detection
Activity assay
 
Handling:Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening. Protect from light.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Contents:25ml Cell Lysis Buffer, 2ml 2x Reaction Buffer, 125µl YVAD-AFC Substrate (1mM), 100µl DTT (1M).
 
Technical Info/Product Notes:YVAD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
 
UniProt ID:P29466
 
Regulatory Status:RUO - Research Use Only
 

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