Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate YVAD-AFC.
Product Specification
| Applications: | Fluorescent detection
Activity assay
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| Handling: | Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening. Protect from light. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 25ml Cell Lysis Buffer, 2ml 2x Reaction Buffer, 125µl YVAD-AFC Substrate (1mM), 100µl DTT (1M). |
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| Technical Info/Product Notes: | YVAD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P29466 |
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