Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate YVAD-pNA.
Product Details
| Applications: | Colorimetric detection
Activity assay
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| Handling: | Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl YVAD-pNA (1mM), 100µl DTT (1M), 25ml Dilution Buffer. |
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| Technical Info/Product Notes: | The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.Historical data has shown that BAFF mediates splenocyte survival and induces monocyte survival.
Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P29466 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol.
12, 774086 (2022),
Application(s): Insect Larval Hemocytes,
Abstract;
Full Text
CDK5 protects from caspase‐induced Ataxin‐3 cleavage and neurodegeneration: J. Liman, et al.; J. Neurochem.
129, 1013 (2014),
Abstract;
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