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CD40L, soluble (mouse) (recombinant) set

ALX-850-075-KI01 1 Set 809.00 USD
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CD40L, soluble (mouse) (recombinant) set image
Figure: CD40L-induced proliferation of murine B cells.Method: Splenocytes from Balb/c mice were purified on B220 magnetic beads using the MACS system. The resulting B cells were put in culture at a density of 70’000 cells/well in a 96-well plate. Cells were activated with the indicated concentrations of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) in the presence of 1µg/ml of Enhancer for Ligands  (Prod. No. ALX-804-034). After 36 hours, 0.5µCi of 3H-Thymidine/well was added. Cells were pulsed for 10 hours, then freeze-thawed, harvested and counted.Note: CD40L-mediated activation of murine B cells requires the presence of a cross-linking enhancer. Stimulation with CD40L alone does not induce proliferation."
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CD40L, soluble (mouse) (recombinant) set image

Product Details

Alternative Name:CD154, TNFSF 5, gp39
Applications:ELISA, FUNC
Application Notes:Functional Application: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of CD40L increases 1'000-fold (stimulation in the ng/ml range) in the presence of the cross-linking enhancer (Prod. No. ALX-804-034).
Species reactivity:Human, Mouse
Specificity:CD40L binds to human and mouse CD40 in an ELISA assay.
Purity:≥90% (SDS-PAGE).
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
Contents:10µg of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity of CD40L at least 1'000-fold.
Formulation:Lyophilized. Contains PBS.
Reconstitution:Reconstitute CD40L with 100µl sterile water and each vial of enhancer with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
Concentration:CD40L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Source:Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
Technical Info/Product Notes:CD40L (Prod. No. ALX-522-070) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.
FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID:P27548 (CD40L)
Regulatory Status:RUO - Research Use Only

Product Literature References

The persistent elimination of B cells responding to blood group A carbohydrates by synthetic group A carbohydrates and B-1 cell differentiation blockade: novel concept in preventing antibody-mediated rejection in ABO-incompatible transplantation: T. Irei, et al.; Blood 110, 4567 (2007), Abstract;
Reciprocal CD40 signals through p38MAPK and ERK-1/2 induce counteracting immune responses: R.K. Mathur, et al.; Nat. Med. 10, 540 (2004), Abstract;

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