APO-BRDU™ Kit is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br‐dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counter staining the total DNA.
Product Details
| Applications: | Flow Cytometry
|
| |
| Quantity: | 50 assays. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Contents: | positive control cells, negative control cells, wash buffer, reaction buffer, TdT enzyme, Br-dUTP, rinse buffer, anti-BrdU mAb - FITC, PI/RNase staining buffer. |
| |
| Technical Info/Product Notes: | Br-dUTP is more readily incorporated into the genome of apoptotic cells than are larger ligands like fluorescein, biotin, etc. This greater incorporation coupled with the use of a fluorescein labeled anti-BrdU monoclonal antibody (Prod. No. ALX-804-197L) gives rise to a stronger signal in flow cytometry. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry
27, 1 (1997), (Review),
Abstract;
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color: X. Li, et al.; Exp. Cell. Res.
222, 28 (1996),
Abstract;
Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation: X. Li & Z. Darzynkiewicz; Cell Prolif.
28, 571 (1995),
Abstract;
Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry
13, 795 (1992),
Abstract;
