Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 


TUNEL assay for the detection of apoptosis by flow cytometry
ALX-850-042-KI01 1 Kit 783.00 USD
Do you need bulk/larger quantities?
APO-BRDU™ Kit is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.

The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br‐dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counter staining the total DNA.

Product Details

Applications:Flow Cytometry
Quantity:50 assays.
Shipping:Blue Ice
Long Term Storage:-20°C
Contents:positive control cells, negative control cells, wash buffer, reaction buffer, TdT enzyme, Br-dUTP, rinse buffer, anti-BrdU mAb - FITC, PI/RNase staining buffer.
Technical Info/Product Notes:Br-dUTP is more readily incorporated into the genome of apoptotic cells than are larger ligands like fluorescein, biotin, etc. This greater incorporation coupled with the use of a fluorescein labeled anti-BrdU monoclonal antibody (Prod. No. ALX-804-197L) gives rise to a stronger signal in flow cytometry.
Regulatory Status:RUO - Research Use Only

Product Literature References

Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry 27, 1 (1997), (Review), Abstract;
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color: X. Li, et al.; Exp. Cell. Res. 222, 28 (1996), Abstract;
Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation: X. Li & Z. Darzynkiewicz; Cell Prolif. 28, 571 (1995), Abstract;
Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry 13, 795 (1992), Abstract;

Related Products

5-Bromodeoxyuridine monoclonal antibody (PRB-1) (fluorescein conjugate) 

Flow Cytometry, ICC | Print as PDF
ALX-804-197L-T060 60 tests Fluorescein 386.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
BrdU Detection kit
By catalog section:


Technical Service
Customer Service

Related Literature

Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

All new literature pieces