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CD20 (human) clonal antibody (E17-P)

ALX-810-209-R100 100 µl 254.00 USD
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Product Details

Immunogen:Synthetic peptide corresponding to a C-terminal sequence of human CD20.
UniProt ID:P11836
Species reactivity:Human
Applications:IHC (FS), IHC (PS)
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100-1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
Protocol:IHC (paraffin sections) Protocol:
  1. Deparaffinize the section in 3 changes of xylene, 5 minutes each
  2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each
  3. Rinse in distilled water
  4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes
  5. Wash in distilled water for 5 minutes
  6. For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in microwave (600W) for 10 minutes
  7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 15 minutes
  8. Rinse in distilled water
  9. Wash in 0.05M TRIS/HCl, pH 7.6, supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes
  10. Incubate the section with primary antibody diluted in buffer A** at the dilution 1:100 for 1 hour in the closed wet chamber
  11. Wash twice 5 minutes with buffer A
  12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB)
  13. Wash twice 5 minutes with buffer A
  14. Apply the chromogen (DAB), 10 minutes
  15. Wash in water for 10 minutes
  16. Stain in hematoxylin for 5 minutes
  17. Wash in water for 10 minutes
  18. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each
  19. Wash the section in 2 changes of xylene for 2 minutes each
  20. Mount the slide for observation
*Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0):
Citric acid (anhydrous) ---------- 1.92g
Distilled water --------------------- 1000ml
Mix to dissolve in 700ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at 4°C for longer storage.
**Direct application of primary antibody in buffer A without previous antigen retrieval is optional.
Regulatory Status:RUO - Research Use Only
CD20 (human) clonal antibody (E17-P) Immunohistochemistry
Figure: Formalin-fixed and paraffin-embedded human tonsil tissue (4μm) stained with cAb to CD20 (human) (Prod. No. ALX-810-209) shows strong positive immunostaining. Kindly performed and provided by Katarína Poliaková, MD and Lubomír Straka, MD, Ph.D. from Clinical Pathology Presov, Ltd., Presov, Slovakia.
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CD20 (human) clonal antibody (E17-P) Immunohistochemistry

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