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United States 

Actin clonal antibody (S12-I)

ALX-810-001-R100 100 µl 554.00 USD
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Product Details

Immunogen:Synthetic peptide corresponding to a C-terminal sequence of human β-actin.
UniProt ID:P60709 (β-actin)
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes α-actin and β-actin.
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:ELISA (1:10,000-1:20,000)
Western Blot (1:2,000; Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100, Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
Regulatory Status:RUO - Research Use Only
Actin clonal antibody (S12-I) Western blot
Figure: Western blot of actin in mouse brain crude lysate (50µg of protein loaded).
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Actin clonal antibody (S12-I) Western blot

Product Literature References

Melatonin Suppresses Autophagy Induced by Clinostat in Preosteoblast MC3T3-E1 Cells: Y.M. Yoo, et al.; Int. J. Mol. Sci. 17, e526 (2016), Application(s): Western blot, Abstract; Full Text

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