Product Details
Clone: | MEM-G/1 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Human recombinant HLA-G denatured heavy chain. |
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UniProt ID: | P17693 |
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Species reactivity: | Human
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Specificity: | Recognizes denatured HLA-G heavy chain. |
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Applications: | IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, stain by standard ABC technique, pretraet by heat retrieval in 0.01 citrate buffer (4 x 2 minutes in microwave oven); recommended starting dilution is 1:60-1:100; incubate 1 hour at room temperature; positive tissue: Human placenta - extravillous cytotrophoblast) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A affinity chromatography. |
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Formulation: | Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
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Handling: | Keep at +4°C. Do not freeze. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | +4°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Immunohistochemistry analysis (AEC visualization) of paraffin embedded section of human lung carcinoma using Prod. No.
ALX-805-704.
The antibody MEM-G/1 detects HLA-G molecules in activated macrophages infiltrating lung carcinomas.
The expression of HLA-G in myelo-monocytic cells infiltrating lung pathologic tissues could alter antigenic presentation and contribute to decrease immune response efficiency, subsequently favoring the progression of tumoral or inflammatory process."
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Product Literature References
A critical look at HLA-G: R. Apps, et al.; Trends Immunol.
29, 313 (2008),
Abstract;
Disulfide bond-mediated dimerization of HLA-G on the cell surface: J.E. Boyson, et al.; PNAS
99, 16180 (2002),
Abstract;
Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules: S. Fournel, et al.; Tissue Antigens
55, 510 (2000),
Abstract;
Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment: G. Frumento, et al.; Tissue Antigens
56, 30 (2000),
Abstract;