Product Details
Clone: | MEM-E/02 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human HLA-E denatured heavy chain. |
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UniProt ID: | P13747 |
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Species reactivity: | Human
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Specificity: | Recognizes denatured heavy chain of human HLA-E. Specificity was confirmed on HLA-G/HLA-E Workshop (Victoria 2002). |
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Crossreactivity: | Does not cross-react with HLA-A, -B, -C or -G. |
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Applications: | IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, recommended starting dilution is 5-10µg/ml; heat antigen retrieval (sodium citrate); incubate 1 hour at room temperature; detection DAB) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A-affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
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Use/Stability: | Stable for at least 6 months when stored at +4°C. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Therapeutic induction of regulatory, cytotoxic CD8+ T cells in multiple sclerosis: D.K. Tennakoon, et al.; J. Immunol.
176, 7119 (2006),
Abstract;
Identification of a human HLA-E-restricted CD8+ T cell subset in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine: R. Salerno-Goncalves, et al.; J. Immunol.
173, 5852 (2004),
Abstract;
Full Text
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules: C. Menier, et al.; Hum. Immunol.
64, 315 (2003),
Abstract;