Recombinant human HLA-G refolded with β2-microglobulin and peptide.
UniProt ID:
P17693
Species reactivity:
Human
Specificity:
Recognizes native HLA-G on the cell surface as well as soluble HLA-G molecule in its β2-microglobulin associated form.
Applications:
ELISA, Flow Cytometry, ICC, IHC (FS), IP
Recommended Dilutions/Conditions:
ELISA (capture; in combination with Prod. No. ALX-804-482) Flow Cytometry (1-5µg/ml; JEG-3 cells could be used as positive control) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:
Purified by precipitation.
Formulation:
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
Shipping:
Blue Ice Not Frozen
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Regulatory Status:
RUO - Research Use Only
Product Literature References
A critical look at HLA-G: R. Apps, et al.; Trends Immunol. 29, 313 (2008), Abstract;
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules: C. Menier, et al.; Hum. Immunol. 64, 315 (2003), Abstract;
HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy: B. Fuzzi, et al.; Eur. J. Immunol. 32, 311 (2002), Abstract;
Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases: C. Pangault, et al.; Hum. Immunol. 63, 83 (2002), Abstract;
Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule: J.M. Lozano, et al.; Aids 16, 347 (2002), Abstract;
Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules: S. Fournel, et al.; Tissue Antigens 55, 510 (2000), Abstract;