Product Details
| Alternative Name: | CD66a/CD66c/CD66d/CD66e/CGM7 |
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| Clone: | D14HD11 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | CEACAM5 partially purified from a perchloric acid extract from liver metastases of colonic tumors. |
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| UniProt ID: | P06731 |
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| Species reactivity: | Human
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| Specificity: | Recognizes CEACAM1, 3, 4, 5 and 6. |
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| Applications: | ELISA, Flow Cytometry, IF, WB
CELISA
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| Recommended Dilutions/Conditions: | Flow cytometry: 1.2 µg/106 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
Western Blot: 4 µg/mL
Immunofluorescence: 1 µg/106 cells
Optimal conditions must be determined inidividually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Quality Control: | Routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector. |
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| Formulation: | Liquid. In PBS, pH 7.2. |
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| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. Overexpression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocytes and dendritic cell activation, receptors of Neisseria species and other bacteria. |
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| Regulatory Status: | RUO - Research Use Only |
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Specificity testing of D14HD11 (Prod. No. ALX-805-080). BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, CEACAM5-CEA-CAM8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG1: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified D14HD11 was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1, CEACAM3, CEACAM4, CEACAM5 and CEACAM6 expressing cells (red curves).
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Product Literature References
The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression: D. Baczynska, et al.; Cell. Mol. Biol. Lett.
8, 471 (2003),
Abstract;
Full Text
Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways: O. Billker, et al.; EMBO J.
21, 560 (2002),
Abstract;
Full Text
Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes: I.C. Boulton & S.D. Gray-Owen; Nat. Immunol.
3, 229 (2002),
Abstract;
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site: S.M. Watt, et al.; Blood
98, 1469 (2001),
Abstract;
Full Text
Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells [...]: P. Muenzner, et al.; J. Biol. Chem.
276, 24331 (2001),
Abstract;
Full Text
Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells: P. Muenzner, et al.; Infect. Immun.
68, 3601 (2000),
Abstract;
Full Text
Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells: J. Guignot, et al.; Infect. Immun.
68, 3554 (2000),
Abstract;
Full Text
CEA-related proteins on human urothelial cell lines of different transformation grades: A. Krop-Watorek, et al.; Cancer Lett.
139, 15 (1999),
Abstract;
Molecular analysis of neisserial Opa protein interactions with the CEA family of receptors: identification of determinants contributing to the differential specificities of binding: A. Popp, et al.; Cell. Microbiol.
1, 169 (1999),
Abstract;
Full Text
CD66 carcinoembryonic antigens mediate interactions between Opa-expressing Neisseria gonorrhoeae and human polymorphonuclear phagocytes: S.D. Gray-Owen, et al.; EMBO J.
16, 3435 (1997),
Abstract;
Full Text
CD66 family Workshop: binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules: F. Grunert, et al.; Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto, et al., eds.), Garland Publishing Inc., New York 1012 (1996),
Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA): P. Jantscheff, et al.; Biomed. Biochim. Acta
50, 1261 (1991),
Abstract;
General Literature References
Carcionembryonic antigen: W. Zimmermann; Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc. New York 459 (2002),
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues: S. Hammarström; Semin. Cancer Biol.
9, 67 (1999), (Review),
Abstract;
Carcinoembryonic antigen gene family: molecular biology and clinical perspectives: J.A. Thompson, et al.; J. Clin. Lab. Anal.
5, 344 (1991), (Review),
Abstract;
