Product Details
Alternative Name: | CD95, APO-1, TNFRSF 6 |
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Clone: | APO-1-1 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Human Fas (CD95; APO-1). |
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UniProt ID: | P25445 |
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Species reactivity: | Human
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Applications: | Flow Cytometry, IHC (FS), FUNC
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Recommended Dilutions/Conditions: | Flow Cytometry (1-2µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | APO-1-1 (IgG1) is an isotype-switch variant of the original clone APO-1-3 (ALX-805-020). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, APO-1-1 is especially useful for immunohistology on cryostat sections and on cytospin preparations. Not suitable for stainings on paraffin sections. Functional Application: Induces apoptosis upon cross-linking. |
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Formulation: | Liquid. In PBS, pH 7.2-7.4. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Blue Ice |
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Long Term Storage: | +4°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cytotoxic efficiency of human CD8+ T cell memory subtypes: A. Knörck, et al.; Front. Immunol.
13, 838484 (2022),
Abstract;
Full Text
Epigenetically Enhanced PDT Induces Significantly Higher Levels of Multiple Extrinsic Pathway Apoptotic Factors than Standard PDT, Resulting in Greater Extrinsic and Overall Apoptosis of Cutaneous T-cell Lymphoma: K.A. Salva, et al.; Photochem. Photobiol.
94, 1058 (2018),
Application(s): Multispectral imaging of immunostained cytopreparations,
Abstract;
Natural killer cells induce distinct modes of cancer cell death: Discrimination, quantification and modulation of apoptosis, necrosis and mixed forms: C.S. Backes, et al.; J. Biol. Chem.
293, 16348 (2018),
Abstract;
Full Text
Specific elimination of effector memory CD4+ T cells due to enhanced Fas signaling complex formation and association with lipid raft microdomains: M. Ramaswamy, et al.; Cell Death Differ.
18, 712 (2011),
Abstract;
Full Text
Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD): H. Wajant, et al.; J. Biol. Chem.
275, 24357 (2000),
Abstract;
Full Text
Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L): D.C. Huang, et al.; PNAS
96, 14871 (1999),
Abstract;
Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells: F. Leithauser, et al.; Lab. Invest.
69, 415 (1993),
Abstract;
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