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FLIP (human) monoclonal antibody (7F10)

ALX-804-961-0100 100 µg 447.00 USD
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Product Specification

Alternative Name:CASPER, CLARP, I-FLICE, Usurpin, CFLAR
Immunogen:Recombinant human FLIP.
UniProt ID:O15519
Source:Purified from ascites.
Species reactivity:Human
Applications:ELISA, Flow Cytometry, IHC, WB
Recommended Dilutions/Conditions:Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with the adapter protein, FADD, and caspase-8 and –10, and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R and TNFR1.
Technical Info/Product Notes:Cited samples:
For an overview on cited samples please click here.
Regulatory Status:RUO - Research Use Only
FLIP (human) monoclonal antibody (7F10) Western blot
Western blot analysis of FLIP (human), mAb (7F10) (Prod. No. ALX-804-961): Lane 1: MW marker, Lane 2&4: HeLa cell lysate, Lane 3&5: Jurkat cell lysate. The two membranes were blotted with 7F10 or NF6 clones at 2µg/ml separately. The result showed they detected very similar pattern in these cell lysates.
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FLIP (human) monoclonal antibody (7F10) Western blot

Product Literature References

3-O-acetylrubianol C (3AR-C) induces RIPK1-dependent programmed cell death by selective inhibition of IKKβ: K. Kang, et al.; FASEB J. 34, 4369 (2020), Abstract;
Co-treatment of birinapant with TRAIL synergistically induces apoptosis by downregulating cFLIP(L) in MDA-MB-453 cell lines: E.J. Park, et al.; Biochem. Biophys. Res. Commun. (2020), Abstract;
Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells: A. Wentao, et al.; J. Exp. Clin. Cancer Res. 38, 181 (2019), Abstract; Full Text
Involvement of up-regulation of death receptors and Bim in Hispolon-mediated TNF-related apoptosis-inducing ligand sensitization in human renal carcinoma: J.M. Yun, et al.; J. Cancer Prev. 24, 155 (2019), Application(s): Western-blot using Caki cells, Abstract; Full Text
The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells: M. Li, et al.; J. Exp. Clin. Cancer Res. 38, 64 (2019), Abstract; Full Text
The transcription factor SP3 drives TNF-α expression in response to Smac mimetics: S.T. Beug, et al.; Sci. Signal. 12, eaat9563 (2019), Abstract;
Acquired Resistance of ER-Positive Breast Cancer to Endocrine Treatment Confers an Adaptive Sensitivity to TRAIL through Posttranslational Downregulation of c-FLIP: L. Piggott, et al.; Clin. Cancer Res. 24, 2452 (2018), Abstract;
Differential expression patterns of the short and long isoform of cFLIP on FasL-mediated apoptosis: H.J. Um, et al.; Oncol. Rep. 39, 2443 (2018), Abstract;
Differential expression patterns of the short and long isoform of cFLIP on FasL‑mediated apoptosis: H.J. Um, et al.; Oncol. Rep. 39, 2443 (2018), Abstract;
Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells: K.K. Kolluri, et al.; Elife 7, e30224 (2018), Abstract; Full Text
Metformin-induced apoptosis facilitates degradation of the cellular caspase 8 (FLICE)-like inhibitory protein through a caspase-dependent pathway in human renal cell carcinoma A498 cells: J.H. Jang, et al.; Oncol. Lett. 16, 2030 (2018), Abstract; Full Text
TRAIL-Induced Apoptosis in TRAIL-Resistant Breast Carcinoma Through Quercetin Cotreatment: J.M. Manouchehri, et al.; Breast Cancer (Auckl.) 12, 1178223417749855 (2018), Application(s): Western blot, Abstract; Full Text
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation: C.M. Henry, et al.; Mol. Cell 65, 715 (2017), Abstract;
cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition: C. Padmanabhan, et al.; Oncotarget 8, 101072 (2017), Abstract; Full Text
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways: M.C. Tanzer, et al.; Cell Death Differ. 24, 481 (2017), Abstract; Full Text
Role of p63 and p73 isoforms on the cell death in patients with hepatocellular carcinoma submitted to orthotopic liver transplantation: R. Gonzalez, et al.; PLoS One 12, e0174326 (2017), Abstract; Full Text
Autophagy-Mediated Degradation of IAPs and c-FLIP(L) Potentiates Apoptosis Induced by Combination of TRAIL and Chal-24: J. Xu, et al.; J. Cell. Biochem. 117, 1136 (2016), Abstract; Full Text
Galangin sensitizes TRAIL-induced apoptosis through down-regulation of anti-apoptotic proteins in renal carcinoma Caki cells: M.A. Han, et al.; Sci. Rep. 6, 18642 (2016), Abstract; Full Text
Infection of epithelial cells with Chlamydia trachomatis inhibits TNF-induced apoptosis at the level of receptor internalization while leaving non-apoptotic TNF-signalling intact: C. Waguia Kontchou, et al.; Cell. Microbiol. 18, 1583 (2016), Abstract;
Overcoming resistance to TRAIL-induced apoptosis in solid tumor cells by simultaneously targeting death receptors, c-FLIP and IAPs: Y. Huang, et al.; Int. J. Oncol. 49, 153 (2016), Application(s): Western-blot using U2OS cells, Abstract; Full Text
Quercetin sensitizes pancreatic cancer cells to TRAIL-induced apoptosis through JNK-mediated cFLIP turnover: J.H. Kim, et al.; Int. J. Biochem. Cell Biol. 78, 327 (2016), Application(s): Western blot; cell lysates, Abstract;
Targeting of BCL2 family proteins with ABT-199 and homoharringtonine reveals BCL2- and MCL1-dependent subgroups of diffuse large B-cell lymphoma: M. Klanova, et al.; Clin. Cancer Res. 22, 1138 (2016), Application(s): Western-blot using BJAB and OCI-Ly7 cells, Abstract;
YM155 sensitizes TRAIL-induced apoptosis through cathepsin S-dependent down-regulation of Mcl-1 and NF-κB-mediated down-regulation of c-FLIP expression in human renal carcinoma Caki cells: S.M. Woo, et al.; Oncotarget 7, 61520 (2016), Abstract; Full Text
6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression: M.A. Han, et al.; Chem. Biol. Interact. 228, 69 (2015), Abstract;
FAS-ligand regulates differential activation-induced cell death of human T-helper 1 and 17 cells in healthy donors and multiple sclerosis patients: M.T. Cencioni, et al.; Cell. Death Dis. 6, e1741 (2015), Application(s): Western Blot, Abstract; Full Text
G2/M Arrest Sensitises Erythroid Leukemia Cells to TRAIL-induced Apoptosis: S. Cruet-Hennequart, et al.; J. Leuk. 3, 174 (2015), Application(s): Western Blot, Full Text
Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis: S. Shi, et al.; Oncotarget 6, 1640 (2015), Abstract; Full Text
Helicobacter pylori sensitizes TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human gastric epithelial cells through regulation of FLIP: W.C. Lin, et al.; Cell Death Dis. 5, e1109 (2014), Application(s): Western blot using human gastric adenocarcinoma cell line AGS, Abstract; Full Text

General Literature References

Cellular FLIP is expressed in cardiomyocytes and down-regulated in TUNEL-positive grafted cardiac tissues: T. Imanishi, et al.; Cardiovasc. Res. 48, 101 (2000), Abstract; Full Text

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