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LRRC32 monoclonal antibody (Plato-1) (FITC conjugate)

ALX-804-867FI-0100 100 µg 541.00 USD
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Product Details

Alternative Name:Leucine-rich repeat-containing protein 32, GARP, Garpin, Glycoprotein A repetitions predominant
Immunogen:Recombinant human LRRC32 (aa 20-627).
UniProt ID:Q14392
Source:Purified from concentrated hybridoma tissue culture supernatant.
Species reactivity:Human, Mouse
Applications:Flow Cytometry
Recommended Dilutions/Conditions:Flow Cytometry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~100kDa by Western blot. Calculated molecular weight of the full-length protein is ~72kDa.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS containing 0.09% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Shipping:Blue Ice Not Frozen
Short Term Storage:+4°C
Long Term Storage:+4°C
Scientific Background:LRRC32 (leucine rich repeat containing 32; also known as GARP or Garpin; Glycoprotein A repetitions predominant) is a glycoprotein expressed on the cell surface of megakaryocytes, platelets and activated regulatory T (Treg) cells.
Regulatory Status:RUO - Research Use Only
LRRC32 monoclonal antibody (Plato-1) (FITC conjugate) Flow Cytometry
Flow cytometry analysis, intracellular staining, of 106 Jurkat cells using LRRC32, mAb (Plato-1) (FITC conjugate) at a concentration of 50μg/ml.
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LRRC32 monoclonal antibody (Plato-1) (FITC conjugate) Flow Cytometry

Product Literature References

Feline glycoprotein A repetitions predominant anchors transforming growth factor beta on the surface of activated CD4(+)CD25(+) regulatory T cells and mediates AIDS lentivirus-induced T cell immunodeficiency: M.M. Miller, et al.; AIDS Res. Hum. Retroviruses 29, 641 (2013), Application(s): Flow cytometry, Abstract; Full Text
Frequency of Circulating Regulatory T Cells Increases during Chronic HIV Infection and Is Largely Controlled by Highly Active Antiretroviral Therapy: P. Presicce, et al.; PLoS One 6, e28118 (2011), Abstract; Full Text
Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein, leucine rich repeat containing 32 (LRRC32): D.V. Chan, et al.; BMC 12, 27 (2011), Abstract; Full Text
Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells: R. Wang, et al.; PNAS 106, 13439 (2009), Abstract;
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells: D.Q. Tran, et al.; PNAS 106, 13445 (2009), Abstract;
Therapeutic potential of FOXP3+ regulatory T cells and their interactions with dendritic cells: D.Q. Tran & E.M. Shevach; Hum. Immunol. 70, 294 (2009), Abstract;

General Literature References

Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression: R. Wang, et al.; PLoS ONE 3, e2705 (2008), Abstract; Full Text

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