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CTRP7 monoclonal antibody (Anna-1)

 
ALX-804-835-C100 100 µg 250.00 USD
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Product Specification

Alternative Name:Complement C1q tumor necrosis factor-related protein 7, C1qTNF7
 
Clone:Anna-1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant mouse CTRP7 (C1q tumor necrosis factor-α-related protein 7) (aa 18-290).
 
UniProt ID:Q8BVD7
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human, Mouse
 
Applications:ELISA, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:200)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Adiponectin is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle. CTRP7 belongs to a highly conserved family of adiponectin paralogs designated as C1q tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. A member of this family, CTRP2 (mouse) has been reported to rapidly induce phosphorylation of AMP-activated protein kinase, acetyl-CoA carboxylase, and mitogen-activated protein kinase in C2C12 myotubes, which resulted in increased glycogen accumulation and fatty acid oxidation.
 
CTRP7 monoclonal antibody (Anna-1) Western blot
Figure 1: Western blot analysis of mouse CTRP7 using MAb to CTRP7 (Anna-1) (Prod. No. ALX-804-835). Method: Cell extracts (5x105) from HEK 293T( lane1) or HEK293wt transfected with a plasmid coding for mouse CTRP7 (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to CTRP7 (Anna-1) (1:1’000) for 2 hours, followed by incubation with HRP-coupled anti-mouse secondary antibody (1:4’000)."
CTRP7 monoclonal antibody (Anna-1) Immunohistochemistry
Figure 2: Immunohistochemical staining of endogenous human CTRP7 in different human tissues (paraffin sections) using MAb to CTRP7 (Anna-1) (Prod. No. ALX-804-835). Method: Different human normal (A: Cerabral cortex; B: Colon; C: Kidney) or cancer (D: Prostate ; E: Colo-rectal; F: Pancreatic) tissues were stained with MAb to CTRP7 (Anna-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (www.proteinatlas.org)."
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CTRP7 monoclonal antibody (Anna-1) Western blot CTRP7 monoclonal antibody (Anna-1) Immunohistochemistry

General Literature References

A family of Acrp30/adiponectin structural and functional paralogs: G.W. Wong, et al.; PNAS 101, 10302 (2004), Abstract;

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