Product Specification
| Alternative Name: | NACHT-, LRR- and PYD-containing protein 3, CIAS1, Cryopyrin, Cold autoinflammatory syndrome 1 protein, PYPAF1, PYRIN-containing APAF1-like protein 1 |
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| Clone: | Nalpy3-b |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human NLRP3/NALP3 (NACHT-, LRR- and PYD-containing protein 3) (pyrin domain). |
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| UniProt ID: | Q96P20 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Specificity: | Detects endogenous protein by IP and WB. |
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| Applications: | IHC (FS), IP, WB
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| Recommended Dilutions/Conditions: | Immunoprecipitation (1:50)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b) (Prod. No.
ALX-804-819).
Method: 5µm thick frozen section of tissue are dried and fixed with acetone. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).
Picture courtesy of Dr. Alain Kummer, University Medical Center Utrecht, The Netherlands."
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Product Literature References
ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism: R. Fang, et al.; Mucosal Immunol. (2019),
Abstract;
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration: C. Kosmidou, et al.; Sci. Rep.
8, 61 (2018),
Abstract;
Full Text
Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states: D. Furman, et al.; Nat. Med.
23, 174 (2017),
Abstract;
Full Text
Hypoxia ameliorates intestinal inflammation through NLRP3/mTOR downregulation and autophagy activation: J. Cosin-Roger, et al.; Nat. Commun.
8, 98 (2017),
Abstract;
Full Text
PTPN22 regulates NLRP3-mediated IL1B secretion in an autophagy-dependent manner: M.R. Spalinger, et al.; Autophagy
13, 1590 (2017),
Abstract;
Full Text
Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early-onset autoinflammatory disease: Q. Zhou, et al.; Nat. Genet.
48, 67 (2016),
Application(s): Immunoblots of total cell lysates,
Abstract;
Sulforaphane exerts its anti-inflammatory effect against amyloid-β peptide via STAT-1 dephosphorylation and activation of Nrf2/HO-1 cascade in human THP-1 macrophages: Y.W. An, et al.; Neurobiol. Aging
38, 1 (2016),
Application(s): Western blot,
Abstract;
Dysregulated production of interleukin-1β upon activation of the NLRP3 inflammasome in patients with familial Mediterranean fever: A. Repa, et al.; Hum. Immunol.
76, 488 (2015),
Application(s): Western Blot,
Abstract;
Enhanced activity of NLRP3 inflammasome in peripheral blood cells of patients with active rheumatoid arthritis: C. Choulaki, et al.; Arthritis Res. Ther.
17, 257 (2015),
Abstract;
Full Text
Mechanism of hepatic inflammation during hepatitis C virus infection: A.A. Negash, et al.; (2013), (Thesis paper),
Application(s): WB, IHC of human liver cells,
Abstract;
NLRP3 inflammasome activation in retinal pigment epithelial cells by lysosomal destabilization: implications for age-related macular degeneration: W.A. Tseng, et al.; Invest. Ophthalmol. Vis. Sci.
54, 110 (2013),
Application(s): Immunohistochemistry of paraffin-embedded tissue,
Abstract;
Full Text
Staphylococcus aureus Activates the NLRP3 Inflammasome in Human and Rat Conjunctival Goblet Cells: V. McGilligan, et al.; PLoS One
8, e74010 (2013),
Application(s): WB, IHC, and Cell culture of rat and human cells,
Abstract;
Full Text
Complement Protein C1q Directs Macrophage Polarization and Limits Inflammasome Activity during the Uptake of Apoptotic cells: M. Benoit, et al.; J. Immunol.
188, 5682 (2012),
Application(s): WB, Cell culture of human lymphocytes and macrophages,
Abstract;
Full Text
Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases: M.T. Shio, et al.; PLoS Pathog.
5, e1000559 (2009),
Abstract;
Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome: A. Lev-Sagie, et al.; Am. J. Obstet. Gynecol.
200, 303 (2009),
Application(s): Immunohistochemistry of paraffin-embedded tissue,
Abstract;
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response: J.A. Kummer, et al.; J. Histochem. Cytochem.
55, 443 (2007),
Abstract;
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity
20, 319 (2004),
Abstract;
General Literature References
Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon and J. Tschopp; Cell
117, 561 (2004),
Abstract;
Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties: W. O'Connor, Jr., et al.; J. Immunol.
171, 6329 (2003),
Abstract;
Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation: M. Chamaillard, et al.; Cell. Microbiol.
5, 581 (2003),
Abstract;
Full Text
