Figure: FACS analysis of cells with MAb to DcTRAIL-R2 (Lucy-1) (Prod. No.
ALX-804-817).
Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse DcTRAIL-R2 (thick line). Cells (5x10
5) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of monoclonal antibody to DcTRAIL-R2 (Lucy-1) (Prod. No.
ALX-804-817). After washing in FACS buffer, PE-conjugated antibody to rat IgG (Prod. No.
ALX-211-053) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry."