Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4)

ALX-804-803-C100 100 µg Inquire for pricing
Do you need bulk/larger quantities?

Product Details

Alternative Name:NACHT-, LRR- and PYD-containing protein 1, DEFCAP, NAC, CARD7, Caspase recruitment domain-containing protein 7
Immunogen:Recombinant human NLRP1/NALP1 (NACHT-, LRR- and PYD-containing protein 1) (pyrin domain).
UniProt ID:Q9C000
Source:Purified from concentrated hybridoma tissue culture supernatant.
Species reactivity:Human
Specificity:Recognizes the pyrin domain (PYD) of NLRP1/NALP1.
Crossreactivity:Does not cross-react with mouse NLRP1/NALP1.
Applications:ICC, IHC (FS), IHC (PS), IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,000)
Immunohistochemistry (paraffin sections, microwave treatment)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4) Western blot
Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.Method: Assembly of the inflammasome was induced by shifting the temperature to 30°C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) (Prod. No. ALX-210-905) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) at 1:1’000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5’000 dilution for ECL detection. For more information about the \"time course assembly of inflammasome\" method see F. Martinon; Mol. Cell. 10, 417 (2002)."
NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4) image
Figure: Detection of NLRP1/NALP1 in 293T cells transfected with a human NLRP1/NALP1 expression plasmid. Left: Phase contrast. Right: Staining with MAb to NLRP1/NALP1 (Nalpy1-4).Method: 293T cells transfected with full length human NLRP1/NALP1 were fixed with 4% paraformaldehyde and stained with 1µg/ml MAb to NLRP1/NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) and then Alexa 488 (1:300 dilution)."
Please mouse over
NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4) Western blot NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4) image

Product Literature References

NLRP1 Functions Downstream of the MAPK/ERK Signaling via ATF4 and Contributes to Acquired Targeted Therapy Resistance in Human Metastatic Melanoma: Z. Zhai, et al.; Pharmaceuticals 14, 23 (2020), Abstract;
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response: J.A. Kummer, et al.; J. Histochem. Cytochem. 55, 443 (2007), Abstract;