Product Details
| Alternative Name: | NACHT-, LRR- and PYD-containing protein 1, DEFCAP, NAC, CARD7, Caspase recruitment domain-containing protein 7 |
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| Clone: | Nalpy1-4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human NLRP1/NALP1 (NACHT-, LRR- and PYD-containing protein 1) (pyrin domain). |
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| UniProt ID: | Q9C000 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Specificity: | Recognizes the pyrin domain (PYD) of NLRP1/NALP1. |
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| Crossreactivity: | Does not cross-react with mouse NLRP1/NALP1. |
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| Applications: | ICC, IHC (FS), IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:1,000)
Immunohistochemistry (paraffin sections, microwave treatment)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.
Method: Assembly of the inflammasome was induced by shifting the temperature to 30°C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) (Prod. No.
ALX-210-905) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) (Prod. No.
ALX-804-803) at 1:1’000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5’000 dilution for ECL detection. For more information about the \"time course assembly of inflammasome\" method see F. Martinon; Mol. Cell.
10, 417 (2002)."
Figure: Detection of NLRP1/NALP1 in 293T cells transfected with a human NLRP1/NALP1 expression plasmid. Left: Phase contrast. Right: Staining with MAb to NLRP1/NALP1 (Nalpy1-4).
Method: 293T cells transfected with full length human NLRP1/NALP1 were fixed with 4% paraformaldehyde and stained with 1µg/ml MAb to NLRP1/NALP1 (Nalpy1-4) (Prod. No.
ALX-804-803) and then Alexa 488 (1:300 dilution)."
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Product Literature References
NLRP1 Functions Downstream of the MAPK/ERK Signaling via ATF4 and Contributes to Acquired Targeted Therapy Resistance in Human Metastatic Melanoma: Z. Zhai, et al.; Pharmaceuticals
14, 23 (2020),
Abstract;
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response: J.A. Kummer, et al.; J. Histochem. Cytochem.
55, 443 (2007),
Abstract;
