Product Details
| Alternative Name: | Transmembrane activator and CAML interactor, TNFRSF 13B, CD267 |
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| Clone: | 8F10 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Rat RBL cells expressing mouse TACI. |
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| UniProt ID: | Q9ET35 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Mouse
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| Applications: | Flow Cytometry
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| Formulation: | Liquid. In PBS. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse TACI (thick line). Cells (5 x 10
5) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of rat anti-mTACI 8F10 MAb. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No.
ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry."
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Product Literature References
CD45 links the B cell receptor with cell survival and is required for the persistence of germinal centers: N.D. Huntington, et al.; Nat. Immunol.
7, 190 (2006),
Abstract;
TACI-BLyS signaling via B-cell-dendritic cell cooperation is required for naive CD8+ T-cell priming in vivo: Y. Diaz-de-Durana, et al.; Blood
107, 594 (2006),
Abstract;
B Cell-Activating Factor Belonging to the TNF Family (BAFF)-R Is the Principal BAFF Receptor Facilitating BAFF Costimulation of Circulating T and B Cells: L.G. Ng, et al.; J. Immunol.
173, 807 (2004),
Abstract;
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol.
172, 812 (2004),
Abstract;
