| ALX-804-667-C100 | 100 µg | 465.00 USD |  |
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| Alternative Name: | TRAIL receptor 3, DcR1, Death Receptor 1, TRID, CD263, TNFRSF 10C, TNF-related apoptosis-inducing ligand receptor 3, Tumor necrosis factor receptor superfamily member 10C |
| Clone: | TR3.06 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human TRAIL-R3. |
| UniProt ID: | O14798 |
| Gene/Protein Identifier: | 8794 (Entrez GeneID) |
| Species reactivity: | Human |
| Crossreactivity: | Does not cross-react with human TRAIL-R1, -R2 or -R4. |
| Applications: | Flow Cytometry, IHC (PS), WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 20µg/ml) Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Protein G-affinity purified. |
| Formulation: | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Use/Stability: | Stable for up to 1 year after receipt when stored at -20°C. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Blue Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Scientific Background: | TRAIL-R3 has a higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.TRAIL-R3 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. It lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand. |
| Protocol: | IHC (paraffin sections) TRAIL-R3 specific staining protocol - Incubate sections in 10mM citrate buffer at pH 6.0 and 99°C for 25min. - Block non-specific binding by incubating sections in blocking solution 1 (PBS containing 20mg/ml BSA, 1mg/ml human IgG) for 20min. - Incubate sections overnight at 4°C in blocking solution 1 using 30μg/ml TRAIL-R3 (human), mAb (TR3.06) (Prod. No. ALX-804-667). - Wash sections twice with PBS buffer and blocking solution 2 (20% normal goat serum) for 20min. - After blocking, incubate with secondary antibody for 30min. at room temperature. - Wash twice with PBS buffer. - Incubate with suitable staining (and counterstaining) substrates. |
| Regulatory Status: | RUO - Research Use Only |
TRAIL-R3 polyclonal antibody |
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| ICC, WB | Print as PDF | ||||||
| ADI-905-216-100 | 100 µg | 478.00 USD | |
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TRAIL-R3 (human) polyclonal antibody |
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| Flow Cytometry, ICC, IHC (PS), WB | Print as PDF | ||||||
| ALX-210-744-C200 | 200 µg | 347.00 USD | |
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TRAIL-R3 (human) monoclonal antibody (HS301) |
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| Flow Cytometry, ICC | Print as PDF | ||||||
| ALX-804-344A-C100 | 100 µg | 378.00 USD | |
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TRAIL-R3 (human) monoclonal antibody (DJR3) |
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| Flow Cytometry | Print as PDF | ||||||
| ALX-804-908-0100 | 100 µg | 255.00 USD | |
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