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TRAIL-R3 (human) monoclonal antibody (TR3.06)

ALX-804-667-C100 100 µg 465.00 USD
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Specially developed for the immunohistochemical detection of TRAIL-R3 in paraffin embedded tissue. Has been shown to detect TRAIL-R3 expressed at endogenous levels in paraffin-embedded tissue in different mammary carcinoma tissues.

Product Details

Alternative Name:TRAIL receptor 3, DcR1, Death Receptor 1, TRID, CD263, TNFRSF 10C, TNF-related apoptosis-inducing ligand receptor 3, Tumor necrosis factor receptor superfamily member 10C
Immunogen:Recombinant human TRAIL-R3.
UniProt ID:O14798
Gene/Protein Identifier:8794 (Entrez GeneID)
Species reactivity:Human
Crossreactivity:Does not cross-react with human TRAIL-R1, -R2 or -R4.
Applications:Flow Cytometry, IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 20µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Use/Stability:Stable for up to 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:TRAIL-R3 has a higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.TRAIL-R3 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. It lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.
Protocol:IHC (paraffin sections) TRAIL-R3 specific staining protocol
- Incubate sections in 10mM citrate buffer at pH 6.0 and 99°C for 25min.
- Block non-specific binding by incubating sections in blocking solution 1 (PBS containing 20mg/ml BSA, 1mg/ml human IgG) for 20min.
- Incubate sections overnight at 4°C in blocking solution 1 using 30μg/ml TRAIL-R3 (human), mAb (TR3.06) (Prod. No. ALX-804-667).
- Wash sections twice with PBS buffer and blocking solution 2 (20% normal goat serum) for 20min.
- After blocking, incubate with secondary antibody for 30min. at room temperature.
- Wash twice with PBS buffer.
- Incubate with suitable staining (and counterstaining) substrates.
Regulatory Status:RUO - Research Use Only

Product Literature References

Prognostic significance of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor expression in patients with breast cancer: T. M. Ganten, et al.; J. Mol. Med. 87, 995 (2009), Abstract;

General Literature References

Differential expression of the TRAIL/TRAIL-receptor system in patients with inflammatory bowel disease: S. Brost, et al.; Pathol. Res. Pract. 206, 43 (2010), Abstract;
TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window: R. Koschny, et al.; Hepatology 45, 649 (2007), Abstract;
Preclinical differentiation between apparently safe and potentially hepatotoxic applications of TRAIL either alone or in combination with chemotherapeutic drugs: T.M. Ganten, et al.; Clin. Cancer Res. 12, 2640 (2006), Abstract; Full Text

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