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HERG1 (human) monoclonal antibody (A12)



ALX-804-652-R300	300 µl	508.00 USD
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Product Specification

Alternative Name:	Erg1, Ether-a-go-go related protein 1, KCNH2, Voltage-gated potassium channel subfamily H member 2, Voltage-gated potassium channel Kv11.1

Clone:	A12

Host:	Mouse

Isotype:	IgG2b

Immunogen:	Synthetic peptide corresponding to aa 575-588 (E⁵⁷⁵QPHMDSRIGWLHN⁵⁸⁸) of the extracellular loop S5-P of HERG1 (human ether-a-go-go related protein 1).

UniProt ID:	Q12809

Species reactivity:	Human

Specificity:	Recognizes the extracellular loop S5-P of HERG1.

Applications:	Flow Cytometry, ICC, IHC (PS), IP

Recommended Dilutions/Conditions:	Flow Cytometry (1:2-1:50) Immunocytochemistry (1:200-1:1,000) Immunohistochemistry (paraffin sections, 1:50-1:200) Immunoprecipitation (3-5µl) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Formulation:	Liquid. Sterile DMEM medium containing 10% bovine calf serum.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Scientific Background:	The ether-a-go-go related gene family of K⁺ channels (HERG/Erg) comprises three members, HERG1/Erg1, HERG2/Erg2, HERG3/Erg3. HERG channel activity is relevant to tumor cell biology. It controls cell proliferation, especially in leukaemias. HERG channels are thus considered diagnostic and prognostic factors in human cancers, as well as targets for anti-neoplastic therapies.

Regulatory Status:	RUO - Research Use Only

HERG1 (human) monoclonal antibody (A12) Immunohistochemistry

Figure 1: Immunohistochemistry on colonic mucosa (left) and adenocarcinoma (right) using MAb to HERG1 (human) (A12) (Prod. No. ALX-804-652), performed without permeabilization."

HERG1 (human) monoclonal antibody (A12) Flow Cytometry

Figure 2: FACS analysis of live intact HEK293-HERG1 transfected cells. Blue: MAb to HERG1 (human) (A12) (Prod. No. ALX-804-652) and goat anti-mouse (FITC)Green: Secondary antibody only."

HERG1 (human) monoclonal antibody (A12) Immunocytochemistry

Figure 3: Immunocytochemistry of HL-1 mouse atrial tumor cells using MAb to HERG1 (human) (A12) (Prod. No. ALX-804-652) (dilution: 1:25).Method: HL-1 cells were blocked with 1% BSA in PBS for 30 min on ice and then incubated with MAb to HERG1 (human) (A12) diluted 1:25 in 1% BSA in PBS for 2 hrs on ice. Cells were then fixed with 2% paraformaldehyde for 30 min at RT and blocked with 5% donkey serum in PBS-T (0.05% tween) for 30 min at RT and then with donkey anti-mouse alexa 488 secondary antibody for 1hr at RT."

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