Product Details
Alternative Name: | cis-Golgi integral membrane protein, Golgi phosphoprotein of 130kDa, GIMPc |
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Clone: | A1/118 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | GPP130 (Golgi phosphoprotein of 130kDa) from Vero monkey cells. |
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UniProt ID: | O00461 |
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Species reactivity: | Human Monkey
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Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:1,000) Immunoprecipitation (2µl) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Affinity purified (HYDRA column) and gel filtrated (FPLC). |
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Formulation: | Lyophilized. |
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Reconstitution: | Reconstitute with 100µl PBS containing 0.1% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain and mediates trafficking in the late endosome-bypass pathway. GPP130 continuously recycles between early Golgi and distal compartments. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem.
279, 47242 (2004),
Abstract;
Full Text
Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol.
142, 377 (1998),
Abstract;
Full Text
The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment: J. Klumperman, et al.; J. Cell Sci.
111, 3411 (1998),
Abstract;
Full Text
Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein: A.D. Linstedt, et al.; Mol. Biol. Cell
8, 1073 (1997),
Abstract;
Full Text