Product Details
| Alternative Name: | p28 BAP31, B cell receptor-associated protein BAP31, BCR-associated protein BAP31, CDM, DXS1357E, 6C6-AG tumor-associated antigen, BCAP31 |
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| Clone: | A1/182 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | BAP31 (BCR associated protein BAP031) from Vero monkey cells. |
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| UniProt ID: | P51572 (human) |
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| Species reactivity: | Human
Monkey
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| Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:1,000)
Immunoprecipitation (2µl)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Lyophilized. Protein G-affinity purified and gel filtrated (FPLC). |
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| Reconstitution: | Reconstitute with 100µl PBS containing 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca2+-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFα: L. Wunderle, et al.; Sci. Rep.
6, 27342 (2016),
Application(s): Immunofluorescence,
Abstract;
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A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue: J. Merulla, et al.; Mol. Biol. Cell
26, 1532 (2015),
Abstract;
Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution: A. Chadrin, et al.; J. Cell. Biol.
189, 795 (2010),
Abstract;
Selective export of human GPI-anchored proteins from the endoplasmic reticulum: C. Bonnon, et al.; J. Cell Sci.
123, 1705 (2010),
Abstract;
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell
19, 1976 (2008),
Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS
102, 6350 (2005),
Abstract;
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Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem.
279, 47242 (2004),
Abstract;
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Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment: D.R. Klopfenstein, et al.; J. Cell Biol.
153, 1287 (2001),
Abstract;
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A novel direct interaction of endoplasmic reticulum with microtubules: D.R. Klopfenstein, et al.; EMBO J.
17, 6168 (1998),
Abstract;
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The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment: J. Klumperman, et al.; J. Cell Sci.
111, 3411 (1998),
Abstract;
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General Literature References
Bap31 Is an Itinerant Protein that Moves between the Peripheral ER and a Juxtanuclear Compartment Related to ER-associated Degradation: Y. Wakana, et al.; Mol. Biol. Cell
19, 1825 (2008),
Abstract;
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Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol: D.G. Breckenridge, et al.; J. Cell. Biol.
160, 1115 (2003),
Abstract;
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Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol.
20, 6731 (2000),
Abstract;
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