Product Details
| Alternative Name: | Macrogolgin, Golgi complex-associated 372kDa protein, GCP372 |
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| Clone: | G1/133 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Giantin from Caco-2 cell Golgi membrane fraction. |
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| UniProt ID: | Q14789 |
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| Species reactivity: | Avian, Mammalian, Monkey
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| Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:1,000)
Immunoprecipitation (2µl)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Not recommended for Western Blot |
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| Purity Detail: | Affinity purified (HYDRA column) and gel filtrated (FPLC). |
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| Formulation: | Lyophilized. Preservative free. |
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| Reconstitution: | Reconstitute with 100µl PBS containing 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ: A. Pagliuso, et al.; Nat. Commun.
7, 12148 (2016),
Application(s): Antibody for immunofluorescence,
Abstract;
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Iterative sorting of apical and basolateral cargo in MDCK cells: A. Treyer, et al.; Mol. Biol. Cell (2016),
Application(s): Immunofluorescence,
Abstract;
Full Text
Substrate Requirements for SPPL2b-dependent Regulated Intramembrane Proteolysis: L. Martin, et al.; J. Biol. Chem.
284, 5662 (2009),
Abstract;
Full Text
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell
19, 1976 (2008),
Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS
102, 6350 (2005),
Abstract;
Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem.
279, 47242 (2004),
Abstract;
Full Text
Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol.
142, 377 (1998),
Abstract;
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Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol.
131, 57 (1995),
Abstract;
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Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa: A.D. Linstedt and H.P. Hauri; Mol. Biol. Cell
4, 679 (1993),
Abstract;
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