Product Details
| Alternative Name: | TLR5, Toll/Interleukin-1 receptor-like gene 3, TIL3 |
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| Clone: | 19D759.2 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Synthetic peptide corresponding to a portion of aa 700-800 of human TLR5 (Toll-like receptor 5). |
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| UniProt ID: | O60602 |
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| Species reactivity: | Human, Mouse
Dog
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| Specificity: | Recognizes an intracellular epitope in the cytoplasmic domain of TLR5. |
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| Applications: | Flow Cytometry, IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (intracellular, 0.5-3µg/1 x 106 cells)
Immunohistochemistry (frozen sections, 10µg/ml)
Immunohistochemistry (paraffin sections, 5µg/ml)
Western Blot (1-3µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Flow Cytometry: Suitable for cell surface and intracellular FC. |
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| Positive Control: | Ramos cells can be used as positive control. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. |
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| Use/Stability: | Stable for 6 months when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Western blot analysis of TLR5 in A) Ramos and B) Raw cell lysate using MAb to Toll-like Receptor 5 (19D759.2) (Prod. No.
ALX-804-598) at 2µg/ml."
Figure 2: Intracellular flow analysis of TLR5 in Ramos cells using 1ug of MAb to Toll-like Receptor 5 (19D759.2) (Prod. No.
ALX-804-598). Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences); orange represents MAb to Toll-like Receptor 5 (19D759.2). Secondary antibody: Goat Anti-Mouse IgG PE."
Figure 3: Intracellular flow analysis of TLR5 in human PBMC (monocytes) using 0.5 ug of MAb to Toll-like Receptor 5 (19D759.2) (Prod. No.
ALX-804-598). Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences); red represents MAb to Toll-like Receptor 5 (19D759.2). Secondary antibody: Goat Anti-Mouse IgG PE."
Figure 4: Immunohistochemical analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype (top) and
to MAb to Toll-like Receptor 5 (19D759.2) (Prod. No.
ALX-804-598) (bottom) at 5μg/ml."
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Product Literature References
The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease: M. Fukata, et al.; J. Immunol.
180, 1886 (2008),
Abstract;
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells: N.K. Crellin, et al.; J. Immunol.
175, 8051 (2005),
Abstract;
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad: S. Francois, et al.; J. Immunol.
174, 3633 (2005),
Abstract;
Full Text
TGF-alpha regulates TLR expression and function on epidermal keratinocytes: L.S. Miller, et al.; J. Immunol.
174, 6137 (2005),
Abstract;
Full Text
