Product Specification
| Alternative Name: | Suppressor of lin-12-like protein |
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| Clone: | MSel1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human SEL1L (suppressor of lin-12-like protein). |
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| UniProt ID: | Q9UBV2 |
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| Species reactivity: | Human, Mouse
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| Applications: | IHC (FS), IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide. |
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| Handling: | Do not freeze. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Scientific Background: | SEL1L (suppressor of lin-12-like protein) is the human ortholog of the C. elegans sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in ubiquitin-dependent degradation of misfolded ER proteins. In addition, SEL1L plays a role in cancer progression. Several studies provided significant evidence that increased SEL1L expression is linked to a decrease in tumor aggressiveness. |
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Figure 2: Pancreatic adenocarcinomas: (b) with and (c) without SEL1L expression.
Figure: Western blot analysis using MAb to SEL1L (MSel1) (Prod. No.
ALX-804-579).Lane 1: MAb to SEL1L (MSel1)Lane 2: Immunoprecipitation of total lysates from BT-474 cells followed by Western blot analysis using MAb to SEL1L (MSel1) (Prod. No. ALX-804-579) (see method below)Lane 3: No lysateLane 4: MW marker.
Method: Cells were lysed using a buffer containing 50mM TRIS-HCl, pH 7.4, 150mM NaCl, 10% glycerol, 0.5% NP40 and protease inhibitors (10µg/ml aproteinin, 1µg/ml leupeptin, 1µg/ml PMSF) for 10 min. at 4°C. Lysates were then centrifuged at 12’000 rpm for 10 min. at 4°C to remove insoluble material. Protein concentration was determined by Comassie Plus Protein Assay (Pierce, Rockford, IL). Protein extracts (1.5mg/sample) were immunoprecipitated for 2 hours at 4°C using 5µg of SEL1L and 20µl of protein-A/G Sepharose beads (Pharmacia-Amersham). Precipitates were then separated on 10% SDS-PAGE, transferred onto nitrocellulose membrane (Amersham) and probed with 10µg/ml MAb to SEL1L (MSel1). Proteins were revealed using ECL-Plus chemiluminescence (Amersham) following the manufacturer’s instructions.
Note: MAb to SEL1L (MSel1) can recognize isoforms of SEL1L protein with MW lower than 88kDa."
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Product Literature References
A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes: M. Kaneko, et al.; FEBS Lett.
581, 5355 (2007),
Abstract;
Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells: M. Schelhaas, et al.; Cell
131, 516 (2007),
Abstract;
SEL1L a multifaceted protein playing a role in tumor progression: I. Biunno, et al.; J. Cell. Physiol.
208, 23 (2006),
Abstract;
SEL1L expression in pancreatic adenocarcinoma parallels SMAD4 expression and delays tumor growth in vitro and in vivo: M. Cattaneo, et al.; Oncogene
22, 6359 (2003),
Abstract;
Production of a monoclonal antibody directed against the recombinant SEL1L protein: R. Orlandi, et al.; J. Biol. Markers
17, 104 (2002),
Abstract;
SEL1L expression decreases breast tumor cell aggressiveness in vivo and in vitro: R. Orlandi, et al.; Cancer Res.
62, 567 (2002),
Abstract;
Full Text
General Literature References
A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes: M. Kaneko, et al.; FEBS Lett.
581, 5355 (2007),
Abstract;
SEL1L a multifaceted protein playing a role in tumor progression: I. Biunno, et al.; J. Cell Physiol.
208, 23 (2006),
Abstract;
Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabditis elegans sel-1: Y. Harada, et al.; J. Hum. Genet.
44, 330 (1999),
Abstract;
