Clone: | FYN-01 |
|
Host: | Mouse |
|
Isotype: | IgG2b |
|
Immunogen: | Recombinant fragment of human Fyn (aa 7-176). |
|
UniProt ID: | P06241 |
|
Species reactivity: | Human, Mouse
|
|
Applications: | ICC, IHC (PS), IP, WB
|
|
Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 5µg/ml) Immunoprecipitation (preparation of cell lysate: 30 min. on ice (orbital incubator) in lysis buffer containing N-dodecyl-β-D-maltoside (Prod. No. ALX-500-007) (20mM TRIS-Cl, 100mM sodium chloride, pH 8.2, 1% laurylmaltoside, 50mM sodium fluoride); positive control J77 T-cell lysate) Western Blot (2µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Application Notes: | Detects a band of ~59kDa by immunoprecipitation. |
|
Purity Detail: | Protein A-affinity purified. |
|
Formulation: | Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
|
Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
|
Shipping: | Blue Ice |
|
Short Term Storage: | +4°C |
|
Long Term Storage: | -20°C |
|
Regulatory Status: | RUO - Research Use Only |
|