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Osteocalcin (undercarboxylated) monoclonal antibody (GluOC4-5)

ALX-804-538-C100 100 µg Inquire for pricing
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Product Details

Immunogen:Synthetic peptide corresponding to aa 14-30 human osteocalcin decarboxylated glutamic acid residues 21 and 24.
UniProt ID:P02818
Source:Obtained by fusing the mouse myeloma cells line P3U1 with splenocytes of BALB/c mouse immunized with KLH-conjugated peptide from human osteocalcin. Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography.
Species reactivity:Human, Rat
Bovine, Goat, Guinea pig, Porcine, Rabbit, Sheep
Specificity:Recognizes human osteocalcin with decarboxylated glutamic acid residues 21 and 24.
Crossreactivity:Does not cross-react with Gla-type osteocalcin.
Applications:ELISA, IHC (PS), WB
Recommended Dilutions/Conditions:ELISA (5-10µg/ml; colorimetric detection; use combination of clones GluOC4-5 (solid phase) and OCG3 (labelled) (Prod. No. ALX-804-536))
Immunohistochemistry (paraffin sections, 5-10µg/ml; Fixation by AMeX modified method)
Western Blot (5-10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservative.
Reconstitution:Dissolve in 50µl distilled water to a final concentration of 2mg/ml.
Dilute with 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
Use/Stability:Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year. 
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

The Effect of Recombinant Undercarboxylated Osteocalcin on Endothelial Dysfunction: T. Qaradakhi, et al.; Calcif. Tissue Int. 105, 546 (2019), Application(s): IHC on rabbit abdominal aorta, Abstract;

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ALX-804-536-C100 100 µg 762.00 USD
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