Product Details
Clone: | GluOC4-5 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 14-30 human osteocalcin decarboxylated glutamic acid residues 21 and 24. |
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UniProt ID: | P02818 |
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Source: | Obtained by fusing the mouse myeloma cells line P3U1 with splenocytes of BALB/c mouse immunized with KLH-conjugated peptide from human osteocalcin. Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography. |
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Species reactivity: | Human, Rat Bovine, Goat, Guinea pig, Porcine, Rabbit, Sheep
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Specificity: | Recognizes human osteocalcin with decarboxylated glutamic acid residues 21 and 24. |
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Crossreactivity: | Does not cross-react with Gla-type osteocalcin. |
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Applications: | ELISA, IHC (PS), WB
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Recommended Dilutions/Conditions: | ELISA (5-10µg/ml; colorimetric detection; use combination of clones GluOC4-5 (solid phase) and OCG3 (labelled) (Prod. No. ALX-804-536)) Immunohistochemistry (paraffin sections, 5-10µg/ml; Fixation by AMeX modified method) Western Blot (5-10µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservative. |
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Reconstitution: | Dissolve in 50µl distilled water to a final concentration of 2mg/ml. Dilute with 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide. |
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Use/Stability: | Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The Effect of Recombinant Undercarboxylated Osteocalcin on Endothelial Dysfunction: T. Qaradakhi, et al.; Calcif. Tissue Int.
105, 546 (2019),
Application(s): IHC on rabbit abdominal aorta,
Abstract;
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