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Osteocalcin (21-31) monoclonal antibody (OCG3)

ALX-804-536-C100 100 µg 762.00 USD
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Product Details

Immunogen:Bovine osteocalcin
UniProt ID:P02820
Species reactivity:Human, Rat
Bovine, Goat, Porcine, Rabbit, Sheep
Specificity:Recognizes aa 21-31 of osteocalcin.
Crossreactivity:Does not cross-react with mouse osteocalcin.
Applications:IHC (FS), IHC (PS), WB
Application Notes:Immunohistochemistry: suitable for frozen sections; weak on paraffin sections
Purity Detail:Purified by column chromatography.
Formulation:Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA and 0.1% sodium azide.
Reconstitution:Reconstitute with 50µl distilled water. Dilute with 10mM TBS*, pH 7.5, containing 1% BSA and 0.1% sodium azide**.
*) TBS should be used for dilution, as the antibody has a tendency to precipitate in PBS.
**) When stored at +4°C, 0.1% sodium azide should be added as a preservative.
Use/Stability:Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 1 year when stored at -20°C and for 6 months when stored at +4°C (after adding 0.1% sodium azide). Be sure to store the antibody at a minimum concentration of 2.0mg/ml. A lower concentration may result in decreased stability. Diluted antibody should not be stored.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Osteocalcin monoclonal antibody (4-9) (OCG4) Recognition sites
Figure: Recognition sites of MAbs to osteocalcin assayed by peptide fragments.
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Osteocalcin monoclonal antibody (4-9) (OCG4) Recognition sites

General Literature References

Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS Study: P. Vergnaud, et al.; J. Clin. Endocrinol. Metab. 82, 719 (1997), Abstract; Full Text
A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies: N. Koyama, et al.; J. Immunol. Methods 139, 17 (1991), Abstract;
Vitamin K-dependent formation of bone Gla protein (osteocalcin) and its function: P.A. Price; Vitam. Horm. 42, 65 (1985), Abstract;
Characterization of a gamma-carboxyglutamic acid-containing protein from bone: P.A. Price, et al.; PNAS 73, 1447 (1976), Abstract;

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