Product Details
Clone: | OCG3 |
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Host: | Mouse |
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Isotype: | IgG3 |
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Immunogen: | Bovine osteocalcin |
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UniProt ID: | P02820 |
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Species reactivity: | Human, Rat Bovine, Goat, Porcine, Rabbit, Sheep
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Specificity: | Recognizes aa 21-31 of osteocalcin. |
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Crossreactivity: | Does not cross-react with mouse osteocalcin. |
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Applications: | IHC (FS), IHC (PS), WB
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Application Notes: | Immunohistochemistry: suitable for frozen sections; weak on paraffin sections |
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Purity Detail: | Purified by column chromatography. |
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Formulation: | Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA and 0.1% sodium azide. |
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Reconstitution: | Reconstitute with 50µl distilled water. Dilute with 10mM TBS*, pH 7.5, containing 1% BSA and 0.1% sodium azide**.
*) TBS should be used for dilution, as the antibody has a tendency to precipitate in PBS.
**) When stored at +4°C, 0.1% sodium azide should be added as a preservative. |
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Use/Stability: | Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 1 year when stored at -20°C and for 6 months when stored at +4°C (after adding 0.1% sodium azide). Be sure to store the antibody at a minimum concentration of 2.0mg/ml. A lower concentration may result in decreased stability. Diluted antibody should not be stored. |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Recognition sites of MAbs to osteocalcin assayed by peptide fragments.
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General Literature References
Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS Study: P. Vergnaud, et al.; J. Clin. Endocrinol. Metab.
82, 719 (1997),
Abstract;
Full Text
A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies: N. Koyama, et al.; J. Immunol. Methods
139, 17 (1991),
Abstract;
Vitamin K-dependent formation of bone Gla protein (osteocalcin) and its function: P.A. Price; Vitam. Horm.
42, 65 (1985),
Abstract;
Characterization of a gamma-carboxyglutamic acid-containing protein from bone: P.A. Price, et al.; PNAS
73, 1447 (1976),
Abstract;