Product Specification
Clone: | OC4-30 |
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Host: | Mouse |
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Isotype: | IgG2a |
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Immunogen: | Bovine osteocalcin γ-carboxylated at aa 17. |
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UniProt ID: | P02820 |
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Species reactivity: | Human, Rat Bovine, Chicken, Dog, Goat, Porcine, Rabbit, Sheep
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Specificity: | Recognizes an epitope which includes the γ-carboxylated residue (aa 17) of γ-carboxylated osteocalcin. |
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Crossreactivity: | Does not cross-react with the heat decarboxylated variant of the protein. |
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Applications: | IHC (FS), IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (frozen and paraffin sections, 10µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Purified by column chromatography. |
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Formulation: | Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. |
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Reconstitution: | Reconstitute in 50µl distilled water. Dilute in 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide. |
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Use/Stability: | After reconstitution, stable for 6 months when stored at 4°C and for 1 year when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Recognition sites of MAbs to osteocalcin assayed by peptide fragments.
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General Literature References
Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS Study: P. Vergnaud, et al.; J. Clin. Endocrinol. Metab.
82, 719 (1997),
Abstract;
Full Text
A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies: N. Koyama, et al.; J. Immunol. Methods
139, 17 (1991),
Abstract;
Vitamin K-dependent formation of bone Gla protein (osteocalcin) and its function: P.A. Price; Vitam. Horm.
42, 65 (1985),
Abstract;
Characterization of a gamma-carboxyglutamic acid-containing protein from bone: P.A. Price, et al.; PNAS
73, 1447 (1976),
Abstract;