Product Details
Alternative Name: | AITRL, TNFSF 18, Glucocorticoid-induced TNF receptor ligand, Activation-inducible TNF-related ligand |
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Clone: | MGTL 10 |
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Host: | Rat |
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Isotype: | IgG2a |
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Immunogen: | Recombinant mouse GITRL. |
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UniProt ID: | Q7TS55 |
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Species reactivity: | Mouse
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Specificity: | Recognizes both natural and recombinant GITRL. |
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Crossreactivity: | Does not cross-react with human GITRL. |
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Applications: | ELISA, Flow Cytometry, WB
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Recommended Dilutions/Conditions: | ELISA (direct and indirect, 1:5,000-1:10,000) Flow Cytometry (1:1,000) Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; incubate 1 hour at room temperature) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Excellent for Flow Cytometry. |
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Formulation: | Liquid. In PBS, pH 7.4. Contains no preservatives. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: RAW264.7 cells (a mouse macrophage cell line, expressing GITRL) were stained significantly with MGTL 10 (Prod No.
ALX-804-518)."
Figure: Mouse GITRL transfected HEK293 cells were stained significantly with MGTL 10 (Prod No.
ALX-804-518). No binding was detected on empty vector transfected cells."
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Product Literature References
Soluble glucocorticoid-induced tumor necrosis factor receptor (sGITR) stimulates osteoclast differentiation in response to receptor activator of NF-kappaB ligand (RANKL) in osteoclast cells: H.H. Shin, et al.; Bone
36, 832 (2005),
Abstract;