| Clone: | 12G6 |
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| Host: | Rat |
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| Isotype: | IgG1 |
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| Immunogen: | p20 fragment of mouse caspase-12 and synthetic peptide corresponding to aa 183-205 (N183KKFDYLFDRDNADTDILNMQEL205) of mouse caspase-12. |
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| UniProt ID: | O08736 |
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| Species reactivity: | Mouse
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| Specificity: | Recognizes an epitope within the p20 fragment of caspase-12. |
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| Crossreactivity: | Does not cross-react with mouse caspase-1 or -11. |
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| Applications: | ELISA, Flow Cytometry, WB
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| Application Notes: | Detects a band of ~51kDa by Western blot. |
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| Formulation: | Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localised to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues; and is expressed at high levels in skeletal muscle, heart, lung and kidney, and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed. |
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| Regulatory Status: | RUO - Research Use Only |
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