Product Details
| Clone: | 4E11 |
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| Host: | Rat |
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| Isotype: | IgG1 |
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| Immunogen: | p20 fragment of mouse caspase-11. |
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| UniProt ID: | P70343 |
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| Species reactivity: | Mouse, Rat
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| Specificity: | The epitope in the p20 fragment. |
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| Applications: | Flow Cytometry, WB
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| Application Notes: | Detects two bands of ~38kDa and ~43kDa by Western blot. |
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| Formulation: | Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7. Human caspase-4, thought to be the homolog of mouse caspase-11, may be an effective therapeutic target for treatment of septic shock. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of endogenous caspase-11 in mouse spleen and lymph node as two bands of 43 and 38 kDa using anti-caspase MAb (4E11). After 16 hours co-culture with LPS 20mg/ml elevated levels of caspase-11 are detected in mouse spleen and lymph node.
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Product Literature References
Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases: M.F. Van Delft, et al.; Cell Death Differ.
17, 821 (2010),
Application(s): WB using mouse thymocytes cell lysate,
Abstract;
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Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood
107, 1872 (2006),
Abstract;
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General Literature References
Caspase-11 gene expression in response to lipopolysaccharide and interferon-gamma requires nuclear factor-kappa B and signal transducer and activator of transcription (STAT) 1: R. Schauvliege, et al.; J. Biol. Chem.
277, 41624 (2002),
Abstract;
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Distinct downstream pathways of caspase-11 in regulating apoptosis and cytokine maturation during septic shock response: S.J. Kang, et al.; Cell Death Differ.
9, 1115 (2002),
Abstract;
Induction of caspase-11 by inflammatory stimuli in rat astrocytes: lipopolysaccharide induction through p38 mitogen-activated protein kinase pathway: J. Hur, et al.; FEBS Lett.
507, 157 (2001),
Abstract;
