Product Details
| Clone: | biG 14 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Monocytes of different species and immunoaffinity purified soluble human CD14. |
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| UniProt ID: | P08571 |
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| Species reactivity: | Human
Bovine, Dog, Guinea pig, Horse, Porcine
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| Specificity: | Recognized epitope in human: aa 39-44. |
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| Applications: | ELISA, Flow Cytometry, WB, FUNC
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| Application Notes: | Functional Application: inhibits binding of LPS to CD14; 1µg/ml MAb inhibits binding of 0.5µg/ml LPS to CD14. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Lyophilized from PBS. Contains no preservatives. |
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| Reconstitution: | Reconstitute with 100µl distilled water. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin. |
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| Technical Info/Product Notes: | Binding titer at human CD14 transfected CHO-cells is more than 10'000. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli: F. Stelter, et al.; Eur J Biochem
243, 100 (1997),
Abstract;
