Product Details
| Alternative Name: | FLICE |
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| Clone: | 3B10 |
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| Host: | Rat |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant mouse caspase-8 p18 subunit. |
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| UniProt ID: | O89110 |
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| Species reactivity: | Mouse
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| Crossreactivity: | Does not cross-react with human caspase-8. |
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| Applications: | ELISA, Flow Cytometry, ICC, WB
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| Application Notes: | ELISA: recombinant mouse caspase-8
Flow Cytometry: overexpressed casepase-8
Excellent for Immunocytochemistry.
Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | For long term storage, aliquot and freeze at -20°C. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Flow cytometry of HEK 293T cells transfected with FLAG tagged mutated mouse caspase-8.Method: MAbs 1G12 and 3B10 both detect overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) by flow cytometry in permeabilized cells. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
Figure 2: MAbs 1G12 and 3B10 both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. Both MAbs to caspase-8 (mouse) do not recognize human caspase-8, whereas endogenous caspase-8 can be efficiently detected in various mouse cell lines. Upon an apoptotic stimulus e.g. by cross-linked rhsFasL both MAbs to caspase-8 (mouse) do also recognize the cleaved active p18 subunit of mouse caspase-8 in addition to the caspase-8 precursor.
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Product Literature References
TLR-stimulated IRAKM activates caspase-8 inflammasome in microglia and promotes neuroinflammation: C. Zhang, et al.; J. Clin. Invest.
128, 5399 (2018),
Abstract;
Full Text
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways: M.C. Tanzer, et al.; Cell Death Differ.
24, 481 (2017),
Abstract;
Full Text
Caspase-8 deficiency in epidermal keratinocytes triggers an inflammatory skin disease: A. Kovalenko, et al.; J. Exp. Med.
206, 2161 (2009),
Abstract;
Full Text
Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium.: M. Shackleton, et al.; Dev. Dyn.
238, 1010 (2009),
Abstract;
Membrane-bound Fas Ligand Only is Essential for Fas-Induced Apoptosis.: L.A. O Reilly, et al.; Nature
461, 659 (2009),
Abstract;
Stimulation of Toll-like receptor 3 and 4 induces interleukin-1β maturation by caspase-8: J. Maelfait, et al.; J. Exp. Med.
205, 1967 (2008),
Abstract;
Full Text
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly; Cell Death Differ.
11, 724 (2004),
Abstract;
