Product Details
Alternative Name: | AhR |
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Clone: | RPT1 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 12-17 (R12KRRKP17) and aa 22-31 (V22KPIPAEGIK31) of mouse AhR (aryl hydrocarbon receptor). |
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UniProt ID: | P30561 |
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Species reactivity: | Human, Mouse, Rat
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Applications: | IF, IHC (PS), IP, WB
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Recommended Dilutions/Conditions: | Immunofluorescence (1:100-1:1,000) Immunohistochemistry (paraffin sections, 1:10-1:100) Western Blot (1:1500-1:2,000). Suggested dilutions/conditions may not be available for all applications. Optimal should be determined individually for each application. |
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Application Notes: | Detects a band of ~95kDa by Western blot. |
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Formulation: | Liquid. Ascites containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor. Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex: H.S. Chen & G.H. Perdew; J. Biol. Chem.
269, 27554 (1994),
Abstract;
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