Product Details
Alternative Name: | Vasodilator-stimulated phosphoprotein |
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Clone: | 5C6 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to a portion of human VASP (vasodilator-stimulated phosphoprotein) phosphorylated at Ser157 conjugated to KLH. |
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UniProt ID: | P50552 |
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Species reactivity: | Human, Mouse
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Crossreactivity: | Does not cross-react with the non-phosphorylated form of VASP. |
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Applications: | ELISA, ICC, IP, WB, FUNC
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Recommended Dilutions/Conditions: | Immunocytochemistry (1-10µg/ml; may tolerate 0.5% formaldehyde fixation) Western Blot> (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Functional Application: monitoring of PKA activity. |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Lyophilized from 1ml of PBS containing 0.09% sodium azide, PEG, and sucrose. |
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Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Technical Info/Product Notes: | The use of MAb to VASP (activated) (Ser157) (5C6) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only! Replacement for ADI-905-768 |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A conjugate of decyltriphenylphosphonium with plastoquinone can carry cyclic adenosine monophosphate, but not cyclic guanosine monophosphate, across artificial and natural membranes: A.M. Firsov, et al.; Biochim. Biophys. Acta
1860, 329 (2018),
Application(s): Western Blot,
Abstract;
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem.
275, 33536 (2000),
Abstract;
Full Text
Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I: A. Smolenski, et al.; J. Biol. Chem.
275, 25723 (2000),
Abstract;
Full Text