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[pSer157]VASP monoclonal antibody (5C6)

ALX-804-403-C100 100 µg 736.00 USD
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Product Details

Alternative Name:Vasodilator-stimulated phosphoprotein
Immunogen:Synthetic peptide corresponding to a portion of human VASP (vasodilator-stimulated phosphoprotein) phosphorylated at Ser157 conjugated to KLH.
UniProt ID:P50552
Species reactivity:Human, Mouse
Crossreactivity:Does not cross-react with the non-phosphorylated form of VASP.
Applications:ELISA, ICC, IP, WB, FUNC
Recommended Dilutions/Conditions:Immunocytochemistry (1-10µg/ml; may tolerate 0.5% formaldehyde fixation)
Western Blot> (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Functional Application: monitoring of PKA activity.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:The use of MAb to VASP (activated) (Ser157) (5C6) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!

Replacement for ADI-905-768

Regulatory Status:RUO - Research Use Only

Product Literature References

A conjugate of decyltriphenylphosphonium with plastoquinone can carry cyclic adenosine monophosphate, but not cyclic guanosine monophosphate, across artificial and natural membranes: A.M. Firsov, et al.; Biochim. Biophys. Acta 1860, 329 (2018), Application(s): Western Blot, Abstract;
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000), Abstract; Full Text
Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I: A. Smolenski, et al.; J. Biol. Chem. 275, 25723 (2000), Abstract; Full Text

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