Alternative Name: | eNOS, NOS III |
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Clone: | 15E2 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to a portion of phosphorylated human endothelial nitric oxide synthase (eNOS; NOS III) conjugated to KLH. |
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UniProt ID: | P29474 |
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Species reactivity: | Human, Mouse
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Crossreactivity: | Does not cross-react with unphosphorylated eNOS (NOS III) or other unrelated serine-phosphorylated proteins. |
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Applications: | ELISA, WB
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Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer: PBS/Tween 20-based blocking and incubation buffer) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Positive Control: | Included. (Prod. No. ALX-840-039) |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol, PEG, sucrose, and 0.09% sodium azide. |
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Use/Stability: | Stable at -20°C up to 1 year. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Technical Info/Product Notes: | Replacement for ADI-905-657 |
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Regulatory Status: | RUO - Research Use Only |
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