Toll-like receptor 8 monoclonal antibody (44C143)



ALX-804-376-C100	100 µg	479.00 USD
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Product Details

Alternative Name:	TLR8, CD288

Clone:	44C143

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to aa 750-850 of the cytoplasmic portion of human TLR8 (Toll-like receptor 8).

UniProt ID:	Q9NR97

Species reactivity:	Human, Mouse

Applications:	Flow Cytometry, IHC (PS), WB

Recommended Dilutions/Conditions:	Flow Cytometry (intracellular, 0.5-5µg per 10⁶cells; cell surface: see Xu et al. (2006) for details) Immunohistochemistry (paraffin sections: 5µg/ml) Western Blot (1-3µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~120kDa by Western blot. Flow Cytometry: Suitable for cell surface and intracellular FC.

Purity Detail:	Protein G-affinity purified.

Formulation:	Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Scientific Background:	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120kDa.

Technical Info/Product Notes:	Replacement for ADI-CSA-825

Regulatory Status:	RUO - Research Use Only

Toll-like receptor 8 monoclonal antibody (44C143) Western blot

Figure: Western blot analysis of TLR8 in cell lysates from 293 transfected with human TLR8 using ALX-804-376 at a dilution of 2µg/ml. "

Toll-like receptor 8 monoclonal antibody (44C143) Immunohistochemistry

Figure: Immunohistochemical analysis of TLR8 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) or MAb to Toll-like Receptor 8 (44C143) (Prod. No. ALX-804-376)."

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Product Literature References

TlR expression profile of human gingival margin-derived stem progenitor cells: K. Fawzy-El-Sayed, et al.; Med. Oral Patol. Oral Cir. Bucal. 21, e30 (2016), Abstract; Full Text

High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production: S. Xu, et al.; Surgery 140, 170 (2006), Abstract;

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