Product Details
Alternative Name: | TLR8, CD288 |
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Clone: | 44C143 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 750-850 of the cytoplasmic portion of human TLR8 (Toll-like receptor 8). |
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UniProt ID: | Q9NR97 |
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Species reactivity: | Human, Mouse
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Applications: | Flow Cytometry, IHC (PS), WB
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Recommended Dilutions/Conditions: | Flow Cytometry (intracellular, 0.5-5µg per 106 cells; cell surface: see Xu et al. (2006) for details) Immunohistochemistry (paraffin sections: 5µg/ml) Western Blot (1-3µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~120kDa by Western blot. Flow Cytometry: Suitable for cell surface and intracellular FC. |
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Purity Detail: | Protein G-affinity purified. |
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Formulation: | Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120kDa. |
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Technical Info/Product Notes: | Replacement for ADI-CSA-825 |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot analysis of TLR8 in cell lysates from 293 transfected with human TLR8 using
ALX-804-376 at a dilution of 2µg/ml. "
Figure: Immunohistochemical analysis of TLR8 in paraffin-embedded formalin-fixed human brain tissue using an isotype control (top) or MAb to Toll-like Receptor 8 (44C143) (Prod. No.
ALX-804-376)."
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Product Literature References
TlR expression profile of human gingival margin-derived stem progenitor cells: K. Fawzy-El-Sayed, et al.; Med. Oral Patol. Oral Cir. Bucal.
21, e30 (2016),
Abstract;
Full Text
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production: S. Xu, et al.; Surgery
140, 170 (2006),
Abstract;
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