Product Details
| Alternative Name: | DNA methyltransferase-3a |
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| Clone: | 64B1446 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant mouse Dnmt3a (DNA methyltransferase-3a). |
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| UniProt ID: | O88508 |
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| Species reactivity: | Mouse
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| Specificity: | Recognizes C-terminal epitope aa 705-908 of mouse Dnmt3a. |
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| Crossreactivity: | Weakly cross-reacts with human Dnmt3a. |
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| Applications: | ICC, IHC, IP, WB
ChIP
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| Recommended Dilutions/Conditions: | Immunocytochemistry (5µg/ml)
Immunohistochemistry (1:400)
Western Blot (2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.05% sodium azide |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot analysis of Dnmt3a using
ALX-804-370 at 2µg/ml against 10µg of 293 cell lysate transfected with either mouse Dnmt3a (lane A) or mouse Dnmt3b (lane B)."
Figure: Immunostaining of Dnmt3a using
ALX-804-370 at 5µg/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells."
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Product Literature References
Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model: J.M. Stempak, et al.; Carcinogenesis
26, 981 (2005),
Abstract;
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2’-deoxycytidine: M. Oka, et al.; Oncogene
24, 3091 (2005),
Abstract;
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system: J. Feng, et al.; J. Neurosci. Res.
79, 734 (2005),
Abstract;
Myc represses transcription through recruitment of DNA methyltransferase corepressor: C. Brenner, et al.; EMBO
24, 336 (2005),
Abstract;
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res.
64, 8184 (2004),
Abstract;
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin: Q. Yan, et al.; Mol. Cell. Biol.
23, 8416 (2003),
Abstract;
Full Text
A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation: T. Chen, et al.; J. Biol. Chem.
277, 38746 (2002),
Abstract;
Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev.
15, 2940 (2001),
Abstract;
Full Text
