Product Details
| Alternative Name: | DNA methyltransferase-1 |
| |
| Clone: | 60B1220.1 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Synthetic peptide corresponding to aa 637-650 of human Dnmt1 (DNA methyltransferase-1). |
| |
| UniProt ID: | P26358 |
| |
| Species reactivity: | Human, Mouse
Zebrafish
|
| |
| Applications: | ICC, IHC (PS), IP, WB
ChIP
|
| |
| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 1-2µg/ml)
Western Blot (0.1-0.5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Purity Detail: | Protein G-affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. |
| |
| Use/Stability: | Stable for 6 months when stored at +4°C. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Short Term Storage: | +4°C |
| |
| Long Term Storage: | -20°C |
| |
| Technical Info/Product Notes: | Replacement for ADI-KAM-TF040 |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure: Western blot analysis for Dnmt1 using
ALX-804-369 at 2µg/ml dilution against 10µg of cell lysates of mouse ES cell."
Figure 2: Immunohistochemistry of Dnmt1 using
ALX-804-369 at 2µg/ml on formalin-fixed paraffin-embedded medullar kidney tissue sections."
Please mouse over
Product Literature References
Interaction of the epigenetic integrator UHRF1 with the MYST domain of TIP60 inside the cell: W. Ashraf, et al.; J. Exp. Clin. Cancer Res.
36, 188 (2017),
Abstract;
Full Text
5-Aza-deoxycytidine induces selective degradation of DNA methyltransferase 1 by a proteasomal pathway that requires the KEN box, bromo-adjacent homology domain, and nuclear localization signal: K. Ghoshal, et al.; Mol. Cell. Biol.
25, 4727 (2005),
Abstract;
An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection: T.S. Lin, et al.; Cancer Lett.
226, 77 (2005),
Abstract;
Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development: I.Y. Park, et al.; J. Cell Biochem.
94, 585 (2005),
Abstract;
Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma: B.I. Srivastava and M.D. Srivastava; Leuk. Res.
29, 771 (2005),
Abstract;
Immunology of cutaneous vasculitis associated with both etanercept and infliximab: M.D. Srivastava, et al.; Scand. J. Immunol.
61, 329 (2005),
Abstract;
Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells: H. Xin, et al.; J. Biol. Chem.
279, 9539 (2004),
Abstract;
Full Text
Induction of DNA methylation and gene silencing by short interfering RNAs in human cells: H. Kawasaki and K. Taira; Nature
431, 211 (2004),
Abstract;
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res.
64, 8184 (2004),
Abstract;
RNA interference-mediated knockdown of DNA methyltransferase 1 leads to promoter demethylation and gene re-expression in human lung and breast cancer cells: M. Suzuki, et al.; Cancer Res.
64, 3137 (2004),
Abstract;
Full Text
Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns: J.M. Craig, et al.; Hum. Mol. Genet.
12, 3109 (2003),
Abstract;
Full Text
Decrease of DNA methyltransferase 1 expression relative to cell proliferation in transitional cell carcinoma: F. Kimura, et al.; Int. J. Cancer
104, 568 (2003),
Abstract;
Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation: Y.W. Leu, et al.; Cancer Res.
63, 6110 (2003),
Abstract;
Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter: Z. Zhang, et al.; Mech. Ageing Dev.
123, 1257 (2002),
Abstract;
Farnesyltransferase inhibitor (L-744,832) restores TGF-beta type II receptor expression and enhances radiation sensitivity in K-ras mutant pancreatic cancer cell line MIA PaCa-2: R.A. Alcock, et al.; Oncogene
21, 7883 (2002),
Abstract;
The Epstein-Barr virus oncogene product, latent membrane protein 1, induces the downregulation of E-cadherin gene expression via activation of DNA methyltransferases: C.N. Tsai, et al.; PNAS
99, 10084 (2002),
Abstract;
Full Text
The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription: R. Santoro, et al.; Nat. Genet.
32, 393 (2002),
Abstract;
Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev.
15, 2940 (2001),
Abstract;
Full Text
