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Toll-like receptor 3 monoclonal antibody (40C1285.6)

ALX-804-362-C100 100 µg 496.00 USD
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Product Details

Alternative Name:TLR3, CD283
Immunogen:Synthetic peptide corresponding to aa 55-70 (VLNLTHNQLRRLPAAN) of human TLR3 (Toll-like receptor 3) cytoplasmic portion.
UniProt ID:O15455
Species reactivity:Human, Mouse
Applications:Flow Cytometry, ICC, IHC (PS), IP, WB
Recommended Dilutions/Conditions:Flow Cytometry (0.5-4µg/106 cells for intracellular staining)
Immunoprecipitation (2µg/106 transfected cells)
Western Blot (1-3µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Toll-like receptor 3 monoclonal antibody (40C1285.6) Western blot
Figure: Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20µg/lane human intestine tissue lysate (lane C).
Toll-like receptor 3 monoclonal antibody (40C1285.6) (FITC conjugate) Immunohistochemistry
Figure: Immunohistochemistry of human gut lumen (longitudinal section, transverse region) using MAb to Toll-like Receptor 3 (human) (40C1285.6) (Prod. No. ALX-804-362) at 10µg/ml. Data courtesy Dr. Elizabeth Furrie, University of Dundee."
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Toll-like receptor 3 monoclonal antibody (40C1285.6) Western blot Toll-like receptor 3 monoclonal antibody (40C1285.6) (FITC conjugate) Immunohistochemistry

Product Literature References

Differential Effects of Nicotine, Alcohol, and Coexposure on Neuroimmune-Related Protein and Gene Expression in Corticolimbic Brain Regions of Rats: C.A. Randall, et al.; ACS Chem. Neurosci. 14, 628 (2023), Abstract;
Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 7668 (2007), Abstract; Full Text
Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 17696 (2007), Abstract; Full Text
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007), Abstract;
Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes: K. Li, et al.; J. Biol. Chem. 280, 16739 (2005), Abstract; Full Text
Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses: T.A. Schneeman, et al.; J. Immunol. 175, 376 (2005), Abstract;
Thyrocytes express a functional toll-like receptor 3: overexpression can be induced by viral infection and reversed by phenylmethimazole and is associated with Hashimoto’s autoimmune thyroiditis: N. Harii, et al.; Mol. Endocrinol. 19, 1231 (2005), Abstract;
Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria: E. Furrie, et al.; Immunology 115, 565 (2005), Abstract;
The effect of innate immunity on autoimmune diabetes and the expression of Toll-like receptors on pancreatic islets: L. Wen, et al.; J. Immunol. 172, 3173 (2004), Abstract;
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003), Abstract;

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Toll-like receptor 3 monoclonal antibody (40C1285.6) (R-PE conjugate) 

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