| ALX-804-358-C100 | 100 µg | 710.00 USD |  |
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| Alternative Name: | Shc transforming protein 1, SH2 domain protein C1, Shc1 |
| Clone: | 6E10 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to aa 33-39 (E33LPpSPSA39) of shc phosphorylated at Ser36 conjugated to KLH. |
| UniProt ID: | P29353 |
| Species reactivity: | Human, Mouse Dog |
| Crossreactivity: | Does not cross-react with non-phosphorylated shc. |
| Applications: | WB |
| Recommended Dilutions/Conditions: | Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 1% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 2 x PBS). Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommended Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Positive Control: | Included. (Prod. No. 840-033-1) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Regulatory Status: | RUO - Research Use Only |
