Product Details
| Clone: | 31A1067 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human caspase-3. |
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| UniProt ID: | P42574 |
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| Species reactivity: | Human, Mouse, Rat
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| Specificity: | Recognizes procaspase-3 and cleaved 17kDa catalytic subunit of caspase-3. Recognizes an epitope in the large (17kDa) domain subunit of caspase-3. |
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| Applications: | IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (2-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS with 0.05% sodium azide. |
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| Use/Stability: | Stable for 6 months when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Western blot analysis for detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3, which generates 17 and 12kDa, larger and smaller catalytic subunit, respectively.
Figure 2: Western blot analysis of Caspase-3 in multiple human tissues using MAb to Caspase-3 (31A1067) (Prod. No.
ALX-804-305) at 2µg/ml. Tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis."
Figure 3: Western blot analysis of Caspase-3 in A) RAW and B) NIH 3T3 using MAb to Caspase-3 (31A1067) (Prod. No.
ALX-804-305) 2µg/ml."
Figure 4: Immunohistochemical analysis of caspase-3 in formalin-fixed, paraffin-embedded human stomach tissue using using MAb to Caspase-3 (31A1067) (Prod. No.
ALX-804-305) at 5 ug/ml. High (top panel) and low magnificaton (bottom panel). Caspase-3 staining is cytoplasmic in some cells and nuclear in others. Caspase-3 in the nucleus is considered to be an indication of active caspase 3. In most cell types and model systems, cells with active caspase-3 are undergoing apoptosis."
Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human breast cancer (top) and normal colon (bottom) stained with Caspase-3, mAb (31A1067) at 4µg/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.
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Protein expression profiling and molecular classification of gastric cancer by the tissue array method: H.S. Lee, et al.; Clin. Cancer Res.
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Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells: F. Jin, et al.; Cancer Res.
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Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma: P.C. Chiang, et al.; J. Hepatol.
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Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles: W. Jian, et al.; Free Radic. Biol. Med.
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Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells: J.C. Lee, et al.; Carcinogenesis
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Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation: L.F. Jarskog, et al.; Am. J. Psychiatry
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The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change: H. Yamaguchi and H.G. Wang; Oncogene
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