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Cystatin A (human) monoclonal antibody (WR 23/2/3/3)

ALX-804-294-C200 200 µg 421.00 USD
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Product Specification

Alternative Name:ACPI, Stefin A
Clone:WR 23/2/3/3
Immunogen:Purified human cystatin A (Stefin A; ACPI).
UniProt ID:P01040
Species reactivity:Human
Crossreactivity:Does not cross-react with cystatin B.
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:10,000-1:20,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. Protein G purified antibody in PBS containing 0.01% thimerosal.
Handling:Do not freeze.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Scientific Background:Cystatin A (acid cysteine proteinase inhibitor; ACPI) is a natural inhibitor of cysteine proteinases and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. Human cystatin A consists of 98 amino acid residues and belongs to cystatin subfamily I (stefins). Among the target enzymes inhibited by cystatin A are cathepsins B, C, H, L, and S, papain and actinidin. Cystatin A has also been detected in infiltrative breast carcinoma.
Regulatory Status:RUO - Research Use Only
Cystatin A (human) monoclonal antibody (WR 23/2/3/3) Immunohistochemistry
Figure: Immunostaining with MAb to cystatin A in infiltrative breast carcinoma.
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Cystatin A (human) monoclonal antibody (WR 23/2/3/3) Immunohistochemistry

General Literature References

The contribution of N-terminal region residues of cystatin A (stefin A) to the affinity and kinetics of inhibition of papain, cathepsin B, and cathepsin L: S. Estrada, et al.; Biochemistry 38, 7339 (1999), Abstract;
Cysteine proteinase inhibitor cystatin A in breast cancer: T. Kuopio, et al.; Cancer Res. 58, 432 (1998), Abstract;
Genomic structure of human cystatin: M. Yamazaki; DNA Seq. 8, 71 (1997), Abstract;
Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum: A. Anastasi, et al.; Biochem. J. 211, 129 (1983), Abstract;

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