Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

β-Catenin monoclonal antibody (8E4)

ALX-804-260-C100 100 µg 569.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ALX-804-260/1

Product Details

Immunogen:Synthetic peptide corresponding to human β-catenin aa 27-37 conjugated to KLH.
UniProt ID:P35222
Species reactivity:Human, Mouse
Specificity:Recognizes an epitope between aa 27-37 and an additional epitope in the C-Terminal region.
Crossreactivity:Weakly cross-reacts with rat β-catenin.
Applications:ELISA, ICC, IHC (PS), WB
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Immunocytochemistry (1-10µg/ml)
Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride., BSA may be used alternatively but milk is not recommended for blocking and dilution!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:For specific detection of dephospho-β-catenin use Prod. No. ALX-804-259.
Positive Control:Included. (Prod. No. ALX-840-022)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
β-Catenin monoclonal antibody (8E4) WB
Figure 2: Detection of endogenous β-Catenin.Whole cell lysates of serum starved tumor cells (20’000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MAb to β-Catenin (8E4) (Prod. No. ALX-804-260/1) (0.5 µg/ml) for 1h at RT and developed by ECL (exp. time: 30s). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D
β-Catenin monoclonal antibody (8E4) Western blot
Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B:
Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7)  (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
Please mouse over
β-Catenin monoclonal antibody (8E4) WB β-Catenin monoclonal antibody (8E4) Western blot

Product Literature References

MiR-23b-3p suppresses epithelial-mesenchymal transition, migration, and invasion of hepatocellular carcinoma cells by targeting c-MET: N.R. Park, et al.; Heliyon 8, e11135 (2022), Abstract;
An aberrant nuclear localization of E-cadherin is a potent inhibitor of Wnt/β-catenin-elicited promotion of the cancer stem cell phenotype: Y. J. Su, et al.; Oncogenesis 4, e157 (2015), Application(s): Western Blot, Abstract; Full Text
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools: M. van Noort, et al.; Blood 110, 2778 (2007), Abstract;
Illegitimate WNT signaling promotes proliferation of multiple myeloma cells: P.W. Derksen, et al.; PNAS 101, 6122 (2004), Abstract;
Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines: E.M. Boon, et al.; Br. J. Cancer 90, 224 (2004), Abstract;
Activation of the canonical beta-catenin pathway by histamine: S.H. Diks, et al.; J. Biol. Chem. 278, 52491 (2003), Abstract;
Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids: E.M. Schmelz, et al.; Cancer Res. 61, 6723 (2001), Abstract;

Related Products

β-Catenin monoclonal antibody (dephospho, aa 35-50) (7A7) 

ELISA, ICC, IHC (PS), IP, WB | Print as PDF
ALX-804-259-C100 100 µg 553.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Catenin Monoclonal antibody
By catalog section:


Technical Service
Customer Service

Related Literature

Integrated solutions for screening Wnt regulators
Integrated solutions for screening Wnt regulators
Download as PDF

Stem Cells
Stem Cells
Download as PDF

All new literature pieces