Replaces Prod. #: ALX-804-260/1
Product Details
| Clone: | 8E4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to human β-catenin aa 27-37 conjugated to KLH. |
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| UniProt ID: | P35222 |
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| Species reactivity: | Human, Mouse
Dog
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| Specificity: | Recognizes an epitope between aa 27-37 and an additional epitope in the C-Terminal region. |
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| Crossreactivity: | Weakly cross-reacts with rat β-catenin. |
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| Applications: | ELISA, ICC, IHC (PS), WB
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| Recommended Dilutions/Conditions: | ELISA (0.05µg/ml)
Immunocytochemistry (1-10µg/ml)
Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride., BSA may be used alternatively but milk is not recommended for blocking and dilution!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | For specific detection of dephospho-β-catenin use Prod. No. ALX-804-259. |
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| Positive Control: | Included. (Prod. No. ALX-840-022) |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: Detection of endogenous β-Catenin.Whole cell lysates of serum starved tumor cells (20’000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with MAb to β-Catenin (8E4) (Prod. No. ALX-804-260/1) (0.5 µg/ml) for 1h at RT and developed by ECL (exp. time: 30s). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D
Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7) (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
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Product Literature References
MiR-23b-3p suppresses epithelial-mesenchymal transition, migration, and invasion of hepatocellular carcinoma cells by targeting c-MET: N.R. Park, et al.; Heliyon
8, e11135 (2022),
Abstract;
An aberrant nuclear localization of E-cadherin is a potent inhibitor of Wnt/β-catenin-elicited promotion of the cancer stem cell phenotype: Y. J. Su, et al.; Oncogenesis
4, e157 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools: M. van Noort, et al.; Blood
110, 2778 (2007),
Abstract;
Illegitimate WNT signaling promotes proliferation of multiple myeloma cells: P.W. Derksen, et al.; PNAS
101, 6122 (2004),
Abstract;
Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines: E.M. Boon, et al.; Br. J. Cancer
90, 224 (2004),
Abstract;
Activation of the canonical beta-catenin pathway by histamine: S.H. Diks, et al.; J. Biol. Chem.
278, 52491 (2003),
Abstract;
Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids: E.M. Schmelz, et al.; Cancer Res.
61, 6723 (2001),
Abstract;
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