β-Catenin monoclonal antibody (dephospho, aa 35-50) (7A7)



ALX-804-259-C100	100 µg	553.00 USD
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Product Details

Alternative Name:	CTNNB

Clone:	7A7

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to aa 35-50 of human β-catenin conjugated to KLH.

UniProt ID:	P35222

Species reactivity:	Human, Mouse Dog

Crossreactivity:	Weakly cross-reacts with rat cytoplasmic dephospho β-catenin.

Applications:	ELISA, ICC, IHC (PS), IP, WB

Recommended Dilutions/Conditions:	ELISA (use at 0.05µg/ml) Immunocytochemistry (1-10µg/ml) Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. BSA may be used alternatively but milk is not recommended for blocking and dilution!) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Positive Control:	Included. (Prod. No. ALX-840-022)

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Reconstitution:	Reconstitute with 1ml water (15 minutes at room temperature).

Use/Stability:	Stable at -80°C up to 1 year, at 4°C up to 3 months.

Handling:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

β-Catenin monoclonal antibody (8E4) Western blot

Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7) (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)

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Product Literature References

A Formylated Hexapeptide Ligand Mimics the Ability of Wnt-5a to Impair Migration of Human Breast Epithelial Cells: A. Säfholm et al.; J. Biol. Chem. 281, 2740 (2006), Abstract;

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