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β-Catenin monoclonal antibody (dephospho, aa 35-50) (7A7)

ALX-804-259-C100 100 µg 553.00 USD
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Product Details

Alternative Name:CTNNB
Immunogen:Synthetic peptide corresponding to aa 35-50 of human β-catenin conjugated to KLH.
UniProt ID:P35222
Species reactivity:Human, Mouse
Crossreactivity:Weakly cross-reacts with rat cytoplasmic dephospho β-catenin.
Applications:ELISA, ICC, IHC (PS), IP, WB
Recommended Dilutions/Conditions:ELISA (use at 0.05µg/ml)
Immunocytochemistry (1-10µg/ml)
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. BSA may be used alternatively but milk is not recommended for blocking and dilution!)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. ALX-840-022)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
β-Catenin monoclonal antibody (8E4) Western blot
Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B:
Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7)  (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
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β-Catenin monoclonal antibody (8E4) Western blot

Product Literature References

A Formylated Hexapeptide Ligand Mimics the Ability of Wnt-5a to Impair Migration of Human Breast Epithelial Cells: A. Säfholm et al.; J. Biol. Chem. 281, 2740 (2006), Abstract;

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β-Catenin (NT/exon 2) monoclonal antibody (7D11) 

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β-Catenin (exon 3) monoclonal antibody (9G2) 

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