Product Specification
| Alternative Name: | FLICE |
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| Clone: | 12F5 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Recombinant human caspase-8. |
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| UniProt ID: | Q14790 |
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| Species reactivity: | Human
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| Specificity: | Recognizes procaspase-8 and active caspase-8. Detects procaspase-8 (p55/54), the intermediate cleavage products (p43/41) and the p18 active subunit of caspase-8 by Western blot. |
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| Crossreactivity: | Does not cross-react with mouse caspase-8. |
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| Applications: | IHC (FS), IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA and 0.01% thimerosal. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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Figure:
(A) Schematic structure of caspase-8 and its cleavage products as detected by anti-caspase-8 (12F5). The antibody is directed against the p18 subunit and detects procaspase-8 (p55/54), the intermediate cleavage products of 43kD and 41kD and the p18 active subunit of caspase-8.
(B) Detection of Fas ligand-induced caspase-8 processing and activation in human Jurkat cells.
Method: Jurkat cells were treated with Fas ligand (100ng/ml). After the indicated time points cell lysates were prepared and separated on a 12.5% SDS-PAGE (2x106 cells/lane) under reducing conditions. Proteins were immunoblotted with anti-caspase-8 12F5 (1µg/ml). Following incubation with peroxidase-conjugated secondary antibodies caspase-8 processing was detected by enhanced chemoluminescent staining. The open arrowhead indicates the two isoforms of procaspase-8 (caspase-8/a and caspase-8/b), which are cleaved into the intermediate forms p43 and p41 and finally processed to the active p18 subunit (filled arrowheads).
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Product Literature References
BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity: A.M. Heijink, et al.; Nat. Commun.
10, 100 (2019),
Abstract;
Full Text
Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages: M. Buscetta, et al.; FASEB J.
34, 1819 (2019),
Abstract;
Full Text
BAX/BAK-Induced Apoptosis Results in Caspase-8-Dependent IL-1β Maturation in Macrophages: D. Chauhan, et al.; Cell Rep.
25, 2354 (2018),
Abstract;
Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations: S.C. Lee, et al.; Cancer Cell
34, 225 (2018),
Abstract;
The poly(ADP-ribose) polymerase inhibitor olaparib induces up-regulation of death receptors in primary acute myeloid leukemia blasts by NF-κB activation: I. Faraoni, et al.; Cancer Lett.
423, 127 (2018),
Abstract;
Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination: M.C. de Almagro, et al.; Cell Death Dis.
6, e1800 (2015),
Application(s): Immunoprecipitation,
Abstract;
Full Text
Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes: E. Kaemmerer, et al.; World J Gastroenterol
21, 4499 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Up-regulation of Bcl-2 during adipogenesis mediates apoptosis resistance in human adipocytes: S.A. Nagel, et al.; Mol. Cell Endocrinol.
382, 368 (2014),
Application(s): WB using human cell lysates,
Abstract;
Prognostic and therapeutic relevance of FLIP and procaspase-8 overexpression in non-small cell lung cancer: J.S. Riley, et al.; Cell Death Dis.
4, e951 (2013),
Application(s): WB using human cell lysates,
Abstract;
Full Text
Cathepsin D primes caspase-8 activation by multiple intra-chain proteolysis: S. Conus, et al.; J. Biol. Chem.
287, 21142 (2012),
Application(s): WB using human cell lysates,
Abstract;
Full Text
Vorinostat/SAHA-induced apoptosis in malignant mesothelioma is FLIP/caspase 8-dependent and HR23B-independent: J.L. Hurwitz, et al.; Eur. J. Cancer
48, 1096 (2012),
Application(s): WB using human cell and tissue lysates,
Abstract;
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood
117, 5953 (2011),
Application(s): WB using human neutrophil lysates,
Abstract;
Full Text
GDP-mannose-4,6-dehydratase (GMDS) deficiency renders colon cancer cells resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor- and CD95-mediated apoptosis by inhibiting complex II formation: K. Moriwaki, et al.; J. Biol. Chem.
286, 43123 (2011),
Application(s): WB using human cell lysates,
Abstract;
Full Text
Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma: B. Gillissen, et al.; J. Cell Biol.
188, 851 (2010),
Abstract;
A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-α-induced Apoptosis: V. Oliveira, et al.; J. Biol. Chem.
283, 13174 (2008),
Abstract;
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ.
15, 1804 (2008),
Abstract;
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica
93, 1169 (2008),
Application(s): WB using human follicular lymphoma lysate,
Abstract;
Full Text
Constitutive caspase activation and impaired death-inducing signaling complex formation in CD95-resistant, long-term activated, antigen-specific T cells: G. Strauss, et al.; J. Immunol.
171, 1172 (2003),
Abstract;
Full Text
An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis: Y. Kim, et al.; J. Biol. Chem.
277, 22320 (2002),
Abstract;
Full Text
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem.
276, 32585 (2001),
Abstract;
Full Text
Trail-induced apoptosis in Type I leukemic cells is not enhanced by overexpression of bax: L. Jia, et al.; BBRC
283, 1037 (2001),
Abstract;
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol.
30, 1769 (2000),
Abstract;
Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD): H. Wajant, et al.; J. Biol. Chem.
275, 24357 (2000),
Abstract;
Full Text
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood
93, 3053 (1999),
Abstract;
Full Text
P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death: D. Ferrari, et al.; FEBS Lett.
447, 71 (1999),
Abstract;
Sendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32): M. Bitzer, et al.; J. Virol.
73, 702 (1999),
Abstract;
Full Text
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med.
188, 979 (1998),
Abstract;
Full Text
