HIF-1α monoclonal antibody (mgc3)



ALX-804-216-R100	100 µl	796.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:	Hypoxia-inducible factor 1α, ARNT-interacting protein

Clone:	mgc3

Host:	Mouse

Isotype:	IgG1

Immunogen:	Human HIF-1α (hypoxia-inducible factor 1α) (aa 530-826).

UniProt ID:	Q16665

Species reactivity:	Human, Mouse Bovine, Porcine, Primate

Crossreactivity:	Does not cross-react with HIF-1β or HIF-2α.

Applications:	ICC, IP, WB Gel Supershift

Recommended Dilutions/Conditions:	Immunocytochemistry (1:200) Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~116kDa by Western blot after hypoxic induction. Gel Supershift Assay: detects also mouse HIF-1α

Formulation:	Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1α rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1β.

Regulatory Status:	RUO - Research Use Only

HIF-1α monoclonal antibody (mgc3) Western blot

Figure A: Western blot using MAb to Hypoxia-inducible Factor 1α (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (25µg) from normoxic (20% O₂) and hypoxic (1% O₂) human HeLa and mouse Hepa1 cells were subjected to SDS-PAGE and HIF-1α was detected in Western blots using MAb mgc3 (1:1’000). Note that mgc3 detects denatured HIF-1α only in human cells in Western blot analysis [1].

Figure B: Electrophoretic mobility shift assay (EMSA) using MAb to Hypoxia-inducible Factor 1α (mgc3) (Prod. No. ALX-804-216).Method: Nuclear extracts (5µg) from normoxic and hypoxic human HeLa and mouse wild-type (Hepa1) and ARNT-mutant (Hepa1C4) hepatoma cells were analyzed by EMSA. MAb mgc3 supershifted the HIF-1 heterodimer in HeLa and Hepa1 cells. No HIF DNA-binding activity was detected in ARNT-mutant Hepa1C4 cells. In contrast to Western blot analysis, mgc3 was able to detect also non-human HIF-1α in EMSA [1,2].

Please mouse over

Product Literature References

Preconditional activation of hypoxia-inducible factors ameliorates ischemic acute renal failure: W.M. Bernhardt, et al.; Am. J. Soc. Nephrol. 17, 1970 (2006), Abstract; Full Text

General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobuilins raised against thy hypoxia-inducible factor 1alpha: G. Camenisch, et al.; FASEB J. 13, 81 (1999), Abstract; Full Text

Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell. Sci. 112, 1203 (1999), Abstract; Full Text