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CFTR monoclonal antibody (CF3)

ALX-804-214-R100 100 µl 722.00 USD
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Product Details

Alternative Name:Cystic fibrosis transmembrane conductance regulator, ABCC7
Immunogen:Synthetic peptide corresponding to aa 103-117(G103RIIASYDPDNKEER117) in the first extracellular loop of human and rabbit CFTR (cystic fibrosis transmembrane conductance regulator). This sequence is highly conserved in mouse, sheep, bovine and Xenopus laevis CFTR.
UniProt ID:P13569
Species reactivity:Human, Mouse
Crossreactivity:Cross-reacts with related proteins in mouse cell line Heb7a that doesn not contain CFTR mRNA.
Applications:ICC, IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:500; staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane. Antibody also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA.)
Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~170kDa by Western blot.
Functional Application: inhibits epithelial uptake of S. typhi in some mouse cell lines.
Formulation:Liquid. Diluted ascites containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (δ F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
Regulatory Status:RUO - Research Use Only

Product Literature References

Defective CFTR-Dependent CREB Activation Results in Impaired Spermatogenesis and Azoospermia: W.M. Xu, et al.; PLoS One 6, e19120 (2011), Application(s): Western Blot on cell culture, rat primary cells, IF on FFPE mouse&human testicle, Abstract; Full Text
Cystic fibrosis transmembrane conductance regulator is vital to sperm fertilizing capacity and male fertility: W.M. Xu, et al.; PNAS 104, 9816 (2007), Abstract;
Production and characterisation of monoclonal and polyclonal antibodies to different regions of thy cystic fibrosis transmembrane conductance regulator: detection of immunologically related proteins: J. Walker, et al.; J. Cell Sci. 108, 2433 (1995), Abstract;

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