PARP-1 monoclonal antibody (F1-23)



ALX-804-211-R050	50 µl	553.00 USD
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Replaces Prod. #: BML-SA251

Product Details

Alternative Name:	Poly(ADP-ribose) polymerase-1

Clone:	F1-23

Host:	Mouse

Isotype:	IgG1

Immunogen:	Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).

UniProt ID:	P18493

Species reactivity:	Human Bovine

Specificity:	Recognizes an epitope in the zinc finger II DNA binding domain of PARP-1.

Crossreactivity:	Does not cross-react with mouse or rat PARP-1.

Applications:	ELISA, ICC, IP, WB, FUNC ChIP

Recommended Dilutions/Conditions:	ELISA: >1:200,000. Dilution buffer: PBS, pH 7.4, 1% non fat dried milk, 0.05% Tween 20. Detectable antigen range: 50ng of PARP-1 Immunocytochemistry: 1:500. Dilution buffer: 50mM TRIS, pH 7.5, 150mM NaCl, 5% milk. Immunoprecipitation: 1:50. Dilution buffer: RiPA buffer Protein G-Sepharose™ Western Blot: 1:5,000. Dilution buffer: PBS, pH 7.4, 5% non fat dried milk, 0.1% Tween 20. See also separate Western blot protocol. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects bands of ~116kDa (intact PARP-1) and ~24kDa (apoptosis-induced cleavage fragment) by Western blot. Functional Application: neutralizes the biological activity of PARP (~80%).

Formulation:	Liquid. Ascites fluid containing 0.02% sodium azide.

Handling:	Avoid freeze/thaw cycles. For long-term storage, aliquot into individual tubes and refreeze at -80°C.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-80°C

Technical Info/Product Notes:	SEPHAROSE is a trademark of GE Healthcare companies.

Regulatory Status:	RUO - Research Use Only

Product Literature References

DNA-dependent SUMO modification of PARP-1: N. Zilio, et al.; DNA Repair (Amst) 12, 761 (2013), Application(s): Western blot, Abstract; Full Text

The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity: K. Ouararhni, et al.; Genes Dev. 20, 3324 (2006), Abstract; Full Text

PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression: A. Augustin, et al.; J. Cell Sci. 116, 1551 (2003), Abstract;

Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997), Abstract;

FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996), Abstract; Full Text

Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995), Abstract; Full Text

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;

Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993), Abstract;

Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;